ArticleActa orthopaedica2016
Detection of bacteria with molecular methods in prosthetic joint infection: sonication fluid better than periprosthetic tissue.
Article in Acta orthopaedica, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers, 1 of them a synthesis that pooled it.
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Who cites it
26 citing papers in PubMed, 1 synthesis or guideline pooled it, 59 citations in OpenAlex.
- Meta-analysis of sonication prosthetic fluid PCR for diagnosing periprosthetic joint infection.PloS one · 2018Pooled it
- The Significance of Sonication in the Detection of Peri-Implant Infections.Antibiotics (Basel, Switzerland) · 2026Article
- Periprosthetic joint infection after arthroplasty: advances and future prospects.Journal of orthopaedic surgery and research · 2025Review
- Microbiologic analysis of 140 patients undergoing revision total knee arthroplasty due to periprosthetic joint infection and aseptic loosening: droplet digital PCR analysis.Journal of orthopaedic surgery and research · 2025Article
- Multiplex PCR test as an intra-operative diagnostic tool for periprosthetic joint infection in presumed aseptic revision hip and knee arthroplasty: a 1-year follow-up study of 200 cases.Journal of bone and joint infection · 2024Article
- The Function of Sonication in the Diagnosis of Periprosthetic Joint Infection After Total Knee Arthroplasty.The archives of bone and joint surgery · 2022Review
- Understanding orthopedic infections through a different perspective: Microcalorimetry growth curves.Experimental and therapeutic medicine · 2022Article
- Sonication of explants enhances the diagnostic accuracy of synovial fluid and tissue cultures and can help determine the appropriate antibiotic therapy for prosthetic joint infections.International orthopaedics · 2022Article
- An update about molecular biology techniques to detect orthopaedic implant-related infections.EFORT open reviews · 2021Review
- Application of leukocyte esterase strip test in the screening of periprosthetic joint infections and prospects of high-precision strips.Arthroplasty (London, England) · 2020Review
- Advantages of 16S rRNA PCR for the diagnosis of prosthetic joint infection.Experimental and therapeutic medicine · 2020Article
- Periprosthetic joint infection in aseptic total hip arthroplasty revision.International orthopaedics · 2020Article
- Sonication Leads to Clinically Relevant Changes in Treatment of Periprosthetic Hip or Knee Joint Infection.Journal of bone and joint infection · 2020Article
- Propidium monoazide-polymerase chain reaction for detection of residual periprosthetic joint infection in two-stage revision.Molecular biology reports · 2019Article
- Culture-negative periprosthetic joint infections.EFORT open reviews · 2019Review
- Utility of 16S rRNA PCR in the Synovial Fluid for the Diagnosis of Prosthetic Joint Infection.Annals of laboratory medicine · 2018Article
- Comparison of culture and broad-range polymerase chain reaction methods for diagnosing periprosthetic joint infection: analysis of joint fluid, periprosthetic tissue, and sonicated fluid.International orthopaedics · 2018Article
- Small-colony variant ofArthroplasty today · 2018Article
- Cost-benefit analysis of antibiofilm microbiological techniques for peri-prosthetic joint infection diagnosis.BMC infectious diseases · 2018Article
- Diagnosis ofArthroplasty today · 2018Article
Corrections and comments
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Authors and funding
4 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Background and purpose - The correct diagnosis of prosthetic joint infection (PJI) can be difficult because bacteria form a biofilm on the surface of the implant. The sensitivity of culture from sonication fluid is better than that from periprosthetic tissue, but no comparison studies using molecular methods on a large scale have been performed. We assessed whether periprosthetic tissue or sonication fluid should be used for molecular analysis. Patients and methods - Implant and tissue samples were retrieved from 87 patients who underwent revision operation of total knee or total hip arthroplasty. Both sample types were analyzed using broad-range (BR-) PCR targeting the 16S rRNA gene. The results were evaluated based on the definition of periprosthetic joint infection from the Workgroup of the Musculoskeletal Infection Society. Results - PJI was diagnosed in 29 patients, whereas aseptic failure was diagnosed in 58 patients. Analysis of sonication fluid using BR-PCR detected bacteria in 27 patients, whereas analysis of periprosthetic tissue by BR-PCR detected bacteria in 22 patients. In 6 of 7 patients in whom BR-PCR analysis of periprosthetic tissue was negative, low-virulence bacteria were present. The sensitivity and specificity values for periprosthetic tissue were 76% and 93%, respectively, and the sensitivity and specificity values for sonication fluid were 95% and 97%. Interpretation - Our results suggest that sonication fluid may be a more appropriate sample than periprosthetic tissue for BR-PCR analysis in patients with PJI. However, further investigation is required to improve detection of bacteria in patients with so-called aseptic failure.
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