ReviewNature methods2016
A practical guide to hydrogels for cell culture.
Review in Nature methods, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 837 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
837 citing papers in PubMed.
- Cell homing within endodontic hydrogels: A scoping review.The Japanese dental science review · 2026Review
- 3D confinement physically regulates cell cycle progression in budding yeast.Nature communications · 2026Article
- Mimicking tumor hypoxia in a glioblastoma-on-a-chip.Materials today. Bio · 2026Article
- Cell-derived lactate triggers local hydrogelation and phenotype remodelling.Nature communications · 2026Article
- Localized Hydrogel-Based Therapeutics: A Strategic Approach to Mitigating Postoperative Recurrence in Breast Cancer.Polymers · 2026Review
- Strain-Accelerated β-Thiolactone Native Chemical Ligation with Kinetic Control Enables Rapid and Selective Hydrogelation for Biofabrication.Journal of the American Chemical Society · 2026Article
- Discrete 2D Material Programming for 3D Shaping and Morphogenesis-Inspired 4D Bioprinting.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- The evaluation of biological effects of artesunate on ovarian cancer using three-dimensional cell culture methods.Cell and tissue banking · 2026Article
- Matrix degradation promotes fibronectin deposition and spatial remodeling in 3D.Cell reports. Physical science · 2026Article
- Adipose-mimetic granular hydrogels uncover biophysical cues driving breast cancer invasion.Cell biomaterials · 2026Article
- Effects of Concentration, Cross-Linking, and Compression on Macromolecular Partitioning in Biological Hydrogels.ACS macro letters · 2026Article
- Engineering Multilayer Poly(ethylene glycol) Hydrogels via Off-Stoichiometry Thiol-Ene Chemistry.ACS applied polymer materials · 2026Article
- Human Cell-Derived Extracellular Matrix Modulates Endothelial Cell Morphology and Metabolism in Response to Fluid Shear Stress.ACS biomaterials science & engineering · 2026Article
- Hydrogels: current biomedical applications and future directions.Molecular biomedicine · 2026Review
- Diarylcyclopropenones: Modular, Tetherable, Light-Triggerable Reservoirs of Carbon Monoxide in Crosslinked Hydrogels.ACS applied bio materials · 2026Article
- Multicomponent Peptide-MXene-Hyaluronic Acid Hydrogel as a Conductive Scaffold for Enhanced Fibroblast Proliferation and Migration Under Electrical Stimulation.Advanced healthcare materials · 2026Article
- Cell-Type-Tailored Hydrogels for Adoptive Cell Therapy in Cancer.Gels (Basel, Switzerland) · 2026Review
- Injectable Hydrogels for the Treatment of Temporomandibular Joint Osteoarthritis: From Tissue-Engineering Scaffolds to Joint Lubricants and Mechanical Buffers.Pharmaceutics · 2026Review
- Development of a 3D collagen type I hydrogel scaffold for modeling hyperglycemia-induced spinal neuronal damage and therapeutic evaluation.Histochemistry and cell biology · 2026Article
- Innovative Sutureless Fabrication Strategy for Tissue-Engineered Pediatric Heart Valves Using an Evolutive Support System.Annals of biomedical engineering · 2026Article
777 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors.
Funding
Abstract
There is growing appreciation of the role that the extracellular environment plays in regulating cell behavior. Mechanical, structural, and compositional cues, either alone or in concert, can drastically alter cell function. Biomaterials, and particularly hydrogels, have been developed and implemented to present defined subsets of these cues for investigating countless cellular processes as a means of understanding morphogenesis, aging, and disease. Although most scientists concede that standard cell culture materials (tissue culture plastic and glass) do a poor job of recapitulating native cellular milieus, there is currently a knowledge barrier for many researchers in regard to the application of hydrogels for cell culture. Here, we introduce hydrogels to those who may be unfamiliar with procedures to culture and study cells with these systems, with a particular focus on commercially available hydrogels.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.