ArticleMolecular cytogenetics2016
The major horse satellite DNA family is associated with centromere competence.
Article in Molecular cytogenetics, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 25 citations in OpenAlex.
- Phased T2T horse and donkey assemblies from a mule reveal peculiar equid centromere evolution.Cell genomics · 2026Article
- Satellite DNA dynamics across phylogenetic scales in ground beetles and other insects.bioRxiv : the preprint server for biology · 2026Article
- Telomere-to-telomere Genome Assembly of Equus kiang (Tibetan wild donkey).Scientific data · 2026Article
- High-quality, haplotype-resolved reference genomes of the Dutch warmblood horse and Friesian horse using trio binning.BMC genomics · 2025Article
- CENP-A and centromere evolution in equids.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2025Review
- CENP-A/CENP-B uncoupling in the evolutionary reshuffling of centromeres in equids.Genome biology · 2025Article
- Review
- The localization of centromere protein A is conserved among tissues.Communications biology · 2023Article
- A Satellite-Free Centromere inInternational journal of molecular sciences · 2023Article
- Robertsonian Fusion and Centromere Repositioning Contributed to the Formation of Satellite-free Centromeres During the Evolution of Zebras.Molecular biology and evolution · 2022Article
- Molecular Dynamics and Evolution of Centromeres in the Genus Equus.International journal of molecular sciences · 2022Review
- Oncogenic lncRNAs alter epigenetic memory at a fragile chromosomal site in human cancer cells.Science advances · 2022Article
- The unique kind of human artificial chromosome: Bypassing the requirement for repetitive centromere DNA.Experimental cell research · 2020Review
- Article
- Ten years of the horse reference genome: insights into equine biology, domestication and population dynamics in the post-genome era.Animal genetics · 2019Review
- CENP-A binding domains and recombination patterns in horse spermatocytes.Scientific reports · 2019Article
- Article
- Conservation, Divergence, and Functions of Centromeric Satellite DNA Families in the Bovidae.Genome biology and evolution · 2019Article
- Birth, evolution, and transmission of satellite-free mammalian centromeric domains.Genome research · 2018Article
- Satellite DNA evolution: old ideas, new approaches.Current opinion in genetics & development · 2018Review
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Authors and funding
9 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundThe centromere is the specialized locus required for correct chromosome segregation during cell division. The DNA of most eukaryotic centromeres is composed of extended arrays of tandem repeats (satellite DNA). In the horse, we previously showed that, although the centromere of chromosome 11 is completely devoid of tandem repeat arrays, all other centromeres are characterized by the presence of satellite DNA. We isolated three horse satellite DNA sequences (37cen, 2P1 and EC137) and described their chromosomal localization in four species of the genus Equus.
resultsIn the work presented here, using the ChIP-seq methodology, we showed that, in the horse, the 37cen satellite binds CENP-A, the centromere-specific histone-H3 variant. The 37cen sequence bound by CENP-A is GC-rich with 221 bp units organized in a head-to-tail fashion. The physical interaction of CENP-A with 37cen was confirmed through slot blot experiments. Immuno-FISH on stretched chromosomes and chromatin fibres demonstrated that the extension of satellite DNA stretches is variable and is not related to the organization of CENP-A binding domains. Finally, we proved that the centromeric satellite 37cen is transcriptionally active.
conclusionsOur data offer new insights into the organization of horse centromeres. Although three different satellite DNA families are cytogenetically located at centromeres, only the 37cen family is associated to the centromeric function. Moreover, similarly to other species, CENP-A binding domains are variable in size. The transcriptional competence of the 37cen satellite that we observed adds new evidence to the hypothesis that centromeric transcripts may be required for centromere function.
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