Evidence map›Paper›PMID 27122636›Full record

ArticleBiology of reproduction2016

MicroRNA-183-96-182 Cluster Regulates Bovine Granulosa Cell Proliferation and Cell Cycle Transition by Coordinately Targeting FOXO1.

Samuel Gebremedhn, Dessie Salilew-Wondim, Michael Hoelker, Franca Rings, Christiane Neuhoff, Ernst Tholen, Karl Schellander, Dawit Tesfaye

Open access · hybridAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Biology of reproduction, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.

0numbers the graph read from it
0cells of the map it votes in
34citing papers in PubMed
4.9field-weighted citation impact, top 5% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

34 citing papers in PubMed, 64 citations in OpenAlex.

  1. Article
  2. Article
  3. Role of miRNAs in Bovine Oocyte Maturation and Reproductive Regulation.International journal of molecular sciences · 2025
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 1 institution in 1 country.

Samuel GebremedhnInstitute of Animal Science, Department of Animal Breeding and Husbandry, University of Bonn, Bonn, Germany.
Dessie Salilew-WondimInstitute of Animal Science, Department of Animal Breeding and Husbandry, University of Bonn, Bonn, Germany.
Michael HoelkerInstitute of Animal Science, Department of Animal Breeding and Husbandry, University of Bonn, Bonn, Germany Teaching and Research Station Frankenforst, Faculty of Agriculture, University of Bonn, Königswinter, Germany Center of Integrated Dairy Research, University of Bonn, Bonn, Germany.
Franca RingsInstitute of Animal Science, Department of Animal Breeding and Husbandry, University of Bonn, Bonn, Germany Teaching and Research Station Frankenforst, Faculty of Agriculture, University of Bonn, Königswinter, Germany.
Christiane NeuhoffInstitute of Animal Science, Department of Animal Breeding and Husbandry, University of Bonn, Bonn, Germany.
Ernst TholenInstitute of Animal Science, Department of Animal Breeding and Husbandry, University of Bonn, Bonn, Germany.
Karl SchellanderInstitute of Animal Science, Department of Animal Breeding and Husbandry, University of Bonn, Bonn, Germany Center of Integrated Dairy Research, University of Bonn, Bonn, Germany.
Dawit TesfayeInstitute of Animal Science, Department of Animal Breeding and Husbandry, University of Bonn, Bonn, Germany Center of Integrated Dairy Research, University of Bonn, Bonn, Germany tesfaye@itw.uni-bonn.de.
University of Bonn · DE

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Large-scale expression profiling of micro-RNAs (miRNAs) in bovine granulosa cells from dominant and subordinate follicles on Day 19 of the estrous cycle revealed enriched micro-RNA-183-96-182 cluster miRNAs in preovulatory dominant follicles that coordinately regulate the forkhead box protein O1 (FOXO1) gene. However, little is known about the role of this cluster in bovine granulosa cell function. We used an in vitro granulosa cell culture model to investigate this role. Granulosa cells aspirated from small growing follicles (3-5 mm in diameter) were cultured in Dulbecco modified Eagle medium/F-12 medium supplemented with fetal bovine serum and transfected with locked nucleic acid-based miRNA mimics, inhibitors, and corresponding negative controls. Overexpression of the miRNA cluster resulted in suppression of FOXO1 mRNA and protein, whereas inhibition of the cluster increased expression of FOXO1 mRNA. Overexpression also increased the relative rate of cell proliferation, whereas inhibition slowed it down. Similarly, the proportion of cells under G0/G1 arrest declined, whereas the ratio of cells in S phase increased in response to miR-183-96-182 overexpression. Selective knockdown of FOXO1 mRNA using anti-FOXO1 small interfering RNA increased the rate of granulosa cell proliferation, decreased the proportion of cells under G0/G1 arrest, and increased the proportion of cells in the S phase of cell cycle. Our data suggest that miR-183-96-182 cluster miRNAs promote proliferation and G1/S transition of bovine granulosa cells by coordinately targeting FOXO1, suggesting a critical role in granulosa cell function. MicroRNA-183-96-182 cluster regulates bovine granulosa cell function by targeting FOXO1 gene.

Indexed as

AnimalsCattleCell CycleCell ProliferationFemaleForkhead Box Protein O1Granulosa CellsMicroRNAsForkhead Box Protein O1MicroRNAscell cyclecell proliferationFOXO1granulosa cellmiR-183-96-182 cluster

Identifiers

PMID27122636
PMCPMC6702798
OpenAlexW2343346283

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.