ArticleScience translational medicine2016
ZP2 peptide beads select human sperm in vitro, decoy mouse sperm in vivo, and provide reversible contraception.
Article in Science translational medicine, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
26 citing papers in PubMed, 50 citations in OpenAlex.
- Molecular interplay between sperm and oocyte: a narrative review.Human reproduction update · 2026Review
- Functional heterogeneity of sperm binding to the ZP2 N terminus across normospermic and infertile phenotypes.Journal of translational medicine · 2026Article
- Dynamic Remodeling of the Zona Pellucida: Implications for Oocyte Competence and Assisted Reproduction.International journal of molecular sciences · 2025Review
- Mutational disruption of transcription factors binding and regulatory networks in a case of unexplained total fertilization failure.Journal of translational medicine · 2025Article
- Cross-species insemination reveals mouse sperm ability to enter and cross the fish micropyle.eLife · 2025Article
- Crossing the barrier or how regulation of ovastacin controls fertilization and translates into clinical phenotypes.iScience · 2025Review
- Disruption of gamete fusion alters the sperm-egg ratio at gamete interaction.Journal of translational medicine · 2024Article
- Basic, translational and clinical studies in reproductive medicine and clinical reproductive sciences.Journal of translational medicine · 2023Article
- New horizons in human sperm selection for assisted reproduction.Frontiers in endocrinology · 2023Review
- Progesterone Promotes In Vitro Maturation of Domestic Dog Oocytes Leading to Successful Live Births.Life (Basel, Switzerland) · 2022Article
- CatSper and its CaM-like CaFASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2022Article
- Proteomics support the threespine stickleback egg coat as a protective oocyte envelope.Molecular reproduction and development · 2021Article
- New Insights into the Mammalian Egg Zona Pellucida.International journal of molecular sciences · 2021Review
- Oolemma Receptors in Mammalian Molecular Fertilization: Function and New Methods of Study.Frontiers in cell and developmental biology · 2021Review
- Identification of Sialyl-Lewis(x)-Interacting Protein on Human Spermatozoa.Frontiers in cell and developmental biology · 2021Article
- Human Zona Pellucida Glycoproteins: Binding Characteristics With Human Spermatozoa and Induction of Acrosome Reaction.Frontiers in cell and developmental biology · 2021Review
- Preclinical contraceptive development for men and women.Biology of reproduction · 2020Review
- ZP4 Is Present in Murine Zona Pellucida and Is Not Responsible for the Specific Gamete Interaction.Frontiers in cell and developmental biology · 2020Article
- Mammalian spermatozoa and cumulus cells bind to a 3D model generated by recombinant zona pellucida protein-coated beads.Scientific reports · 2019Article
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5 authors at 2 institutions in 1 country.
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Abstract
Gamete recognition in the female reproductive tract occurs at the surface of the zona pellucida surrounding ovulated eggs. The acellular zona matrix is composed of three (mouse) or four (human) proteins (ZP1 to ZP4), and the amino terminus of ZP2 is the primary sperm-binding ligand. Mouse and human sperm bind, respectively, to recombinant moZP2(35-149) and huZP2(39-154) peptides attached to agarose beads. Mouse ZP2 peptide beads markedly inhibited fertilization of ovulated mouse eggs inseminated in vitro and incubated overnight. Similarly, human ZP2 peptide beads prevented sperm binding and penetration of transgenic ZP2(Rescue) zonae pellucidae, in which human ZP2 replaced mouse ZP2. When mouse ZP2 peptide beads were transcervically deposited into the uterus, there was no change in mating behavior and copulatory plugs were present, but bound sperm did not progress into the oviduct and female mice were infertile. On average, contraception lasted >10 estrus cycles but was reversible with no detectable pathology in the reproductive tract. Despite the long-term contraceptive effect, initial sperm binding to the peptide beads was reversible in vitro. We exploited this observation to select human sperm that were better able to penetrate the zonae of human ZP2(Rescue) eggs, and the approach holds promise for identifying superior sperm for human assisted reproductive technologies (ART). We conclude that the amino-terminal ZP2 peptide supports sperm binding, which is initially reversible but, with time, becomes irreversible. Short-term, reversible binding may be useful in selecting sperm for ART, and long-term binding decoys sperm and results in effective contraception in mice.
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