ArticleJournal of experimental botany2016
Improved analysis of C4 and C3 photosynthesis via refined in vitro assays of their carbon fixation biochemistry.
Article in Journal of experimental botany, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 38 papers.
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Who cites it
38 citing papers in PubMed, 99 citations in OpenAlex.
- Rubisco dark inhibition in angiosperms shows a complex distribution pattern.Journal of experimental botany · 2026Article
- Advancements in photosynthetic efficiency: Pathways, regulation, and biotechnological applications for enhancing crop productivity.Plant signaling & behavior · 2025Review
- The metabolite transporters of C4 photosynthesis.The Plant cell · 2025Review
- Laboratory evolution of Rubisco solubility and catalytic switches to enhance plant productivity.Nature plants · 2025Article
- Updated values for Table 1 of fastest rubisco carboxylation rates in Davidi et al 2020.The EMBO journal · 2025Article
- Systems analysis of long-term heat stress responses in the C4 grass Setaria viridis.The Plant cell · 2025Article
- Genetic engineering of RuBisCO by multiplex CRISPR editing small subunits in rice.Plant biotechnology journal · 2025Article
- Improving Crop Yield through Increasing Carbon Gain and Reducing Carbon Loss.Plants (Basel, Switzerland) · 2024Review
- Supplementation of Jasmonic acid Mitigates the Damaging Effects of Arsenic Stress on Growth, Photosynthesis and Nitrogen Metabolism in Rice.Rice (New York, N.Y.) · 2024Article
- Photosynthesis in newly developed leaves of heat-tolerant wheat acclimates to long-term nocturnal warming.Journal of experimental botany · 2024Article
- Seed bio-priming enhanced salt stress tolerance of maize (3 Biotech · 2023Article
- Grafting Rhodobacter sphaeroides with red algae Rubisco to accelerate catalysis and plant growth.Nature plants · 2023Article
- Producing fast and active Rubisco in tobacco to enhance photosynthesis.The Plant cell · 2023Article
- Effects of leaf age during drought and recovery on photosynthesis, mesophyll conductance and leaf anatomy in wheat leaves.Frontiers in plant science · 2023Article
- Removal of redox-sensitive Rubisco Activase does not alter Rubisco regulation in soybean.Photosynthesis research · 2022Article
- Faster than expected Rubisco deactivation in shade reduces cowpea photosynthetic potential in variable light conditions.Nature plants · 2022Article
- Distinct CPlant direct · 2021Article
- Sucrose Utilization for Improved Crop Yields: A Review Article.International journal of molecular sciences · 2021Review
- Magnesium Application Promotes Rubisco Activation and Contributes to High-Temperature Stress Alleviation in Wheat During the Grain Filling.Frontiers in plant science · 2021Article
- Ozone tolerant maize hybrids maintain Rubisco content and activity during long-term exposure in the field.Plant, cell & environment · 2020Article
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Authors and funding
4 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Plants operating C3 and C4 photosynthetic pathways exhibit differences in leaf anatomy and photosynthetic carbon fixation biochemistry. Fully understanding this underpinning biochemical variation is requisite to identifying solutions for improving photosynthetic efficiency and growth. Here we refine assay methods for accurately measuring the carboxylase and decarboxylase activities in C3 and C4 plant soluble protein. We show that differences in plant extract preparation and assay conditions are required to measure NADP-malic enzyme and phosphoenolpyruvate carboxylase (pH 8, Mg(2+), 22 °C) and phosphoenolpyruvate carboxykinase (pH 7, >2mM Mn(2+), no Mg(2+)) maximal activities accurately. We validate how the omission of MgCl2 during leaf protein extraction, lengthy (>1min) centrifugation times, and the use of non-pure ribulose-1,5-bisphosphate (RuBP) significantly underestimate Rubisco activation status. We show how Rubisco activation status varies with leaf ontogeny and is generally lower in mature C4 monocot leaves (45-60% activation) relative to C3 monocots (55-90% activation). Consistent with their >3-fold lower Rubisco contents, full Rubisco activation in soluble protein from C4 leaves (<5min) was faster than in C3 plant samples (<10min), with addition of Rubisco activase not required for full activation. We conclude that Rubisco inactivation in illuminated leaves primarily stems from RuBP binding to non-carbamylated enzyme, a state readily reversible by dilution during cellular protein extraction.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.