ArticleJournal of experimental botany2016
Life without complex I: proteome analyses of an Arabidopsis mutant lacking the mitochondrial NADH dehydrogenase complex.
Article in Journal of experimental botany, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 47 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
47 citing papers in PubMed, 108 citations in OpenAlex.
- Multi-Omics Dataset of Heat Stress-Induced Memory in Arabidopsis.Scientific data · 2026Article
- Fungal Carbonic Anhydrases: A Systematic Review from Molecular Profiling to Pathogenic Regulation inJournal of fungi (Basel, Switzerland) · 2026Review
- Carbonic anhydrases contribute to mitochondrial function, conidial development, and pathogenicity ofApplied and environmental microbiology · 2026Article
- Cytochrome c oxidase inactivation in Physcomitrium patens reveals that respiration coordinates plant metabolism.The Plant cell · 2025Article
- Roles of microRNAs and histone modifications in enhancing stress tolerance in soybean and their applications in molecular breeding.Breeding science · 2025Article
- The complex I subunit B22 contains a LYR domain that is crucial for an interaction with the mitochondrial acyl carrier protein SDAP1.The Plant journal : for cell and molecular biology · 2025Article
- Plant supercomplex I + III2 structure and function: implications for the growing field.Biochemical Society transactions · 2024Review
- Overexpression of RPOTmp Being Targeted to Either Mitochondria or Chloroplasts inInternational journal of molecular sciences · 2024Article
- Deep proteomics reveals incorporation of unedited proteins into mitochondrial protein complexes in Arabidopsis.Plant physiology · 2024Article
- Comparative transcriptome and coexpression network analysis reveals key pathways and hub candidate genes associated with sunflower (Helianthus annuus L.) drought tolerance.BMC plant biology · 2024Article
- Physiological relevance, localization and substrate specificity of the alternative (type II) mitochondrial NADH dehydrogenases ofFrontiers in microbiology · 2024Article
- Arabidopsis Tubby domain-containing F-box proteins positively regulate immunity by modulating PI4Kβ protein levels.The New phytologist · 2023Article
- Quantitative Acetylome Analysis of Differentially Modified Proteins in Virulence-DifferentiatedJournal of fungi (Basel, Switzerland) · 2023Article
- Regulation of Mitochondrial Respiration by Hydrogen Sulfide.Antioxidants (Basel, Switzerland) · 2023Review
- FTSH PROTEASE 3 facilitates Complex I degradation through a direct interaction with the Complex I subunit PSST.The Plant cell · 2023Article
- The biogenesis and regulation of the plant oxidative phosphorylation system.Plant physiology · 2023Review
- Defining the lipidome of Arabidopsis leaf mitochondria: Specific lipid complement and biosynthesis capacity.Plant physiology · 2023Article
- Article
- Proteome-wide identification of non-histone lysine methylation in tomato during fruit ripening.Journal of advanced research · 2022Article
- Transcriptome Analysis to Identify Responsive Genes under Sublethal Concentration of Bifenazate in the Diamondback Moth,International journal of molecular sciences · 2022Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The mitochondrial NADH dehydrogenase complex (complex I) is of particular importance for the respiratory chain in mitochondria. It is the major electron entry site for the mitochondrial electron transport chain (mETC) and therefore of great significance for mitochondrial ATP generation. We recently described an Arabidopsis thaliana double-mutant lacking the genes encoding the carbonic anhydrases CA1 and CA2, which both form part of a plant-specific 'carbonic anhydrase domain' of mitochondrial complex I. The mutant lacks complex I completely. Here we report extended analyses for systematically characterizing the proteome of the ca1ca2 mutant. Using various proteomic tools, we show that lack of complex I causes reorganization of the cellular respiration system. Reduced electron entry into the respiratory chain at the first segment of the mETC leads to induction of complexes II and IV as well as alternative oxidase. Increased electron entry at later segments of the mETC requires an increase in oxidation of organic substrates. This is reflected by higher abundance of proteins involved in glycolysis, the tricarboxylic acid cycle and branched-chain amino acid catabolism. Proteins involved in the light reaction of photosynthesis, the Calvin cycle, tetrapyrrole biosynthesis, and photorespiration are clearly reduced, contributing to the significant delay in growth and development of the double-mutant. Finally, enzymes involved in defense against reactive oxygen species and stress symptoms are much induced. These together with previously reported insights into the function of plant complex I, which were obtained by analysing other complex I mutants, are integrated in order to comprehensively describe 'life without complex I'.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.