ArticleJournal of clinical microbiology2016
Use of Shotgun Metagenome Sequencing To Detect Fecal Colonization with Multidrug-Resistant Bacteria in Children.
Article in Journal of clinical microbiology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
21 citing papers in PubMed, 33 citations in OpenAlex.
- Microbiota restoration reduces antibiotic-resistant bacteria gut colonization in patients with recurrent Clostridioides difficile infection from the open-label PUNCH CD study.Genome medicine · 2021Trial
- Improving Oral Health and Modulating the Oral Microbiome to Reduce Bloodstream Infections from Oral Organisms in Pediatric and Young Adult Hematopoietic Stem Cell Transplantation Recipients: A Randomized Controlled Trial.Biology of blood and marrow transplantation : journal of the American Society for Blood and Marrow Transplantation · 2020Trial
- Article
- Microbiota from young mice restore the function of aged ISCs.Stem cell reports · 2026Article
- Evaluation of a Shotgun Metagenomics Approach for Detection of ESBL- and/or Carbapenemase-ProducingMicroorganisms · 2023Article
- Detection ofFrontiers in microbiology · 2023Article
- Targeted metagenomics using bait-capture to detect antibiotic resistance genes in retail meat and seafood.Frontiers in microbiology · 2023Article
- Article
- Prospective Comparison Between Shotgun Metagenomics and Sanger Sequencing of the 16S rRNA Gene for the Etiological Diagnosis of Infections.Frontiers in microbiology · 2022Article
- Plant resistome profiling in evolutionary old bog vegetation provides new clues to understand emergence of multi-resistance.The ISME journal · 2021Article
- The Diagnostic Value of Metagenomic Next-Generation Sequencing in Lower Respiratory Tract Infection.Frontiers in cellular and infection microbiology · 2021Review
- Article
- Shotgun Metagenomic Detection of Pathogens: a Micro-Comic Strip.Journal of clinical microbiology · 2018Article
- Article
- Leptin receptor q223r polymorphism influences neutrophil mobilization after Clostridium difficile infection.Mucosal immunology · 2018Article
- The antibiotic resistome and microbiota landscape of refugees from Syria, Iraq and Afghanistan in Germany.Microbiome · 2018Article
- Antibiotic Resistance Acquisition in the First Week of Life.Frontiers in microbiology · 2018Article
- Microbial Disruption Indices to Detect Colonization With Multidrug-Resistant Organisms.Infection control and hospital epidemiology · 2017Article
- Network of microbial and antibiotic interactions drive colonization and infection with multidrug-resistant organisms.Proceedings of the National Academy of Sciences of the United States of America · 2017Article
- Metagenomic characterization of the effect of feed additives on the gut microbiome and antibiotic resistome of feedlot cattle.Scientific reports · 2017Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors at 1 institution in 1 country.
Funding
Abstract
Prevention of multidrug-resistant (MDR) bacterial infections relies on accurate detection of these organisms. We investigated shotgun metagenome sequencing for the detection of methicillin-resistant Staphylococcus aureus (MRSA), vancomycin-resistant Enterococcus (VRE), and MDR Enterobacteriaceae Fecal metagenomes were analyzed from high-risk inpatients and compared to those of low-risk outpatients and controls with minimal risk for a MDR bacterial infection. Principal-component analysis clustered patient samples into distinct cohorts, confirming that the microbiome composition was significantly different between cohorts (P = 0.006). Microbial diversity and relative anaerobe abundance were preserved in outpatients compared to those in controls. Relative anaerobe abundance was significantly reduced in inpatients compared to that in outpatients (P = 0.006). Although the potential for MDR bacteria was increased in inpatients and outpatients compared to that in controls (P < 0.001), there was no difference between inpatients and outpatients. However, 9 (53%) inpatients had colonization with a MDR bacterium that was not identified by culture. Unlike culture, shotgun sequencing quantitatively characterizes the burdens of multiple MDR bacteria relative to all of the microbiota within the intestinal community. We propose consideration of key microbiome features, such as diversity and relative anaerobe abundance, in addition to the detection of MDR bacteria by shotgun metagenome sequencing as a novel method that might better identify patients who are at increased risk of a MDR infection.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.