ArticleMolecular microbiology2016
Evolved plasmid-host interactions reduce plasmid interference cost.
Article in Molecular microbiology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 45 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
45 citing papers in PubMed, 74 citations in OpenAlex.
- Host-dependent effects ofFrontiers in microbiology · 2026Article
- Not So Mosaic After All? The Core Genomes of IncP-1 Plasmids Evolve Predominantly Through Vertical Transmission.bioRxiv : the preprint server for biology · 2025Article
- Flavomycin inhibits plasmid-mediated conjugative transfer of antibiotic resistance genes by disrupting energy metabolism and pilus assembly.Zoological research · 2025Article
- Evolution of a Plasmid Regulatory Circuit Ameliorates Plasmid Fitness Cost.Molecular biology and evolution · 2025Article
- The fitness connection of antibiotic resistance.Frontiers in microbiology · 2025Article
- Compensatory evolution of chromosomes and plasmids counteracts the plasmid fitness cost.Ecology and evolution · 2024Article
- Evolution of a Plasmid Regulatory Circuit Ameliorates Plasmid Fitness Cost.bioRxiv : the preprint server for biology · 2024Article
- RecA-dependent or independent recombination of plasmid DNA generates a conflict with the host EcoKI immunity by launching restriction alleviation.Nucleic acids research · 2024Article
- A mathematician's guide to plasmids: an introduction to plasmid biology for modellers.Microbiology (Reading, England) · 2023Review
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- The fitness cost of horizontally transferred and mutational antimicrobial resistance inFrontiers in microbiology · 2023Review
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- Hitherto-Unnoticed Self-Transmissible Plasmids Widely Distributed among Different Environments in Japan.Applied and environmental microbiology · 2022Article
- Contrasting long-term dynamics of antimicrobial resistance and virulence plasmids inMicrobial genomics · 2022Article
- Colonization of gut microbiota by plasmid-carrying bacteria is facilitated by evolutionary adaptation to antibiotic treatment.The ISME journal · 2022Article
- Plasmid Viability Depends on the Ecological Setting of Hosts within a Multiplasmid Community.Microbiology spectrum · 2022Article
- Experimental evolution of the megaplasmid pMPPla107 inPhilosophical transactions of the Royal Society of London. Series B, Biological sciences · 2022Article
- Persistence of plasmid andFrontiers in microbiology · 2022Article
- Let Me Upgrade You: Impact of Mobile Genetic Elements on Enterococcal Adaptation and Evolution.Journal of bacteriology · 2021Review
- Plasmid fitness costs are caused by specific genetic conflicts enabling resolution by compensatory mutation.PLoS biology · 2021Article
Corrections and comments
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Authors and funding
8 authors at 3 institutions in 3 countries.
Funding
Abstract
Antibiotic selection drives adaptation of antibiotic resistance plasmids to new bacterial hosts, but the molecular mechanisms are still poorly understood. We previously showed that a broad-host-range plasmid was poorly maintained in Shewanella oneidensis, but rapidly adapted through mutations in the replication initiation gene trfA1. Here we examined if these mutations reduced the fitness cost of TrfA1, and whether this was due to changes in interaction with the host's DNA helicase DnaB. The strains expressing evolved TrfA1 variants showed a higher growth rate than those expressing ancestral TrfA1. The evolved TrfA1 variants showed a lower affinity to the helicase than ancestral TrfA1 and were no longer able to activate the helicase at the oriV without host DnaA. Moreover, persistence of the ancestral plasmid was increased upon overexpression of DnaB. Finally, the evolved TrfA1 variants generated higher plasmid copy numbers than ancestral TrfA1. The findings suggest that ancestral plasmid instability can at least partly be explained by titration of DnaB by TrfA1. Thus under antibiotic selection resistance plasmids can adapt to a novel bacterial host through partial loss of function mutations that simultaneously increase plasmid copy number and decrease unfavorably high affinity to one of the hosts' essential proteins.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.