ArticleBioMed research international2016
Next Generation Sequencing Identifies Five Major Classes of Potentially Therapeutic Enzymes Secreted by Lucilia sericata Medical Maggots.
Article in BioMed research international, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed, 42 citations in OpenAlex.
- From pre-oral secretions to gut digestion: How do Lucilia sericata (Diptera: Calliphoridae) larvae handle Leishmania major?PloS one · 2025Article
- RETRACTED: Maggot extract accelerates skin wound healing of diabetic rats via enhancing STAT3 signaling.PloS one · 2024Article
- [Influence of maggot excretions/secretions on the anti-Zhonghua shao shang za zhi = Zhonghua shaoshang zazhi = Chinese journal of burns · 2021Article
- Practical context of enzymatic treatment for wound healing: A secreted protease approach (Review).Biomedical reports · 2020Review
- European Medicinal Leeches-New Roles in Modern Medicine.Biomedicines · 2020Review
- New Insights Into Culturable and Unculturable Bacteria Across the Life History of Medicinal MaggotsFrontiers in microbiology · 2020Article
- Expression of a New Recombinant Collagenase Protein ofIranian journal of biotechnology · 2019Article
- Profiling antimicrobial peptides from the medical maggot Lucilia sericata as potential antibiotics for MDR Gram-negative bacteria.The Journal of antimicrobial chemotherapy · 2019Article
- Pharmacological Properties of the Medical Maggot: A Novel Therapy Overview.Evidence-based complementary and alternative medicine : eCAM · 2018Review
- Sarconesin:Frontiers in microbiology · 2018Article
Corrections and comments
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Authors and funding
6 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Lucilia sericata larvae are used as an alternative treatment for recalcitrant and chronic wounds. Their excretions/secretions contain molecules that facilitate tissue debridement, disinfect, or accelerate wound healing and have therefore been recognized as a potential source of novel therapeutic compounds. Among the substances present in excretions/secretions various peptidase activities promoting the wound healing processes have been detected but the peptidases responsible for these activities remain mostly unidentified. To explore these enzymes we applied next generation sequencing to analyze the transcriptomes of different maggot tissues (salivary glands, gut, and crop) associated with the production of excretions/secretions and/or with digestion as well as the rest of the larval body. As a result we obtained more than 123.8 million paired-end reads, which were assembled de novo using Trinity and Oases assemblers, yielding 41,421 contigs with an N50 contig length of 2.22 kb and a total length of 67.79 Mb. BLASTp analysis against the MEROPS database identified 1729 contigs in 577 clusters encoding five peptidase classes (serine, cysteine, aspartic, threonine, and metallopeptidases), which were assigned to 26 clans, 48 families, and 185 peptidase species. The individual enzymes were differentially expressed among maggot tissues and included peptidase activities related to the therapeutic effects of maggot excretions/secretions.
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