Evidence map›Paper›PMID 27118725›Full record

ArticleAnalytical chemistry2016

Multistep Compositional Remodeling of Supported Lipid Membranes by Interfacially Active Phosphatidylinositol Kinases.

Seyed R Tabaei, Feng Guo, Florentine U Rutaganira, Setareh Vafaei, Ingrid Choong, Kevan M Shokat, Jeffrey S Glenn, Nam-Joon Cho

Open access · greenAbstract readLetter
PubMed Full text at PMC Publisher
In one paragraph

Article in Analytical chemistry, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
1.4field-weighted citation impact, top 18% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed, 13 citations in OpenAlex.

  1. Article
  2. PI4KIIIβ-Mediated Phosphoinositides Metabolism Regulates Function of the VTA Dopaminergic Neurons and Depression-Like Behavior.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2024
    Article
  3. Article
  4. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 5 institutions in 2 countries.

Seyed R TabaeiSchool of Materials Science and Engineering and Centre for Biomimetic Sensor Science, Nanyang Technological University , 50 Nanyang Avenue, 639798, Singapore.
Feng GuoDepartments of Medicine, Division of Gastroenterology and Hepatology, and Microbiology & Immunology, Stanford University School of Medicine , Stanford, California 94305, United States.
Florentine U RutaganiraHoward Hughes Medical Institute, Department of Cellular and Molecular Pharmacology, University of California San Francisco , San Francisco, California 94158-2280, United States.
Setareh VafaeiSchool of Materials Science and Engineering and Centre for Biomimetic Sensor Science, Nanyang Technological University , 50 Nanyang Avenue, 639798, Singapore.
Ingrid ChoongDepartments of Medicine, Division of Gastroenterology and Hepatology, and Microbiology & Immunology, Stanford University School of Medicine , Stanford, California 94305, United States.
Kevan M ShokatHoward Hughes Medical Institute, Department of Cellular and Molecular Pharmacology, University of California San Francisco , San Francisco, California 94158-2280, United States.
Jeffrey S GlennDepartments of Medicine, Division of Gastroenterology and Hepatology, and Microbiology & Immunology, Stanford University School of Medicine , Stanford, California 94305, United States.
Nam-Joon ChoSchool of Materials Science and Engineering and Centre for Biomimetic Sensor Science, Nanyang Technological University , 50 Nanyang Avenue, 639798, Singapore.
Nanyang Technological University · SGStanford University · USHoward Hughes Medical Institute · USPalo Alto Veterans Institute for Research · USUniversity of California, San Francisco · US

Funding

Translational Incubator CoreU19AI109662 · NIAID · STANFORD UNIVERSITY · PI GLENN, JEFFREY S · 2014 to 2019
$28.6M
RESEARCH TRAINING IN CHEMISTRY AND CHEMICAL BIOLOGYT32GM064337 · NIGMS · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · PI CRAIK, CHARLES SCOTT · 2002 to 2021
$7.8M
The PIP2-virus interface and PI 4-kinase: novel biology and validation targetsR01AI099245 · NIAID · STANFORD UNIVERSITY · PI GLENN, JEFFREY S · 2012 to 2016
$5.2M
NIAID NIH HHS R01 AI099245NIAID NIH HHS U19 AI109662NIGMS NIH HHS T32 GM064337
6 · The paper itself

Abstract

The multienzyme catalytic phosphorylation of phosphatidylinositol (PI) in a supported lipid membrane platform is demonstrated for the first time. One-step treatment with PI 4-kinase IIIβ (PI4Kβ) yielded PI 4-phosphate (PI4P), while a multistep enzymatic cascade of PI4Kβ followed by PIP 5-kinase produced PI-4,5-bisphosphate (PI(4,5)P2 or PIP2). By employing quartz crystal microbalance with dissipation monitoring, we were able to track membrane association of kinase enzymes for the first time as well as detect PI4P and PI(4,5)P2 generation based on subsequent antibody binding to the supported lipid bilayers. Pharmacologic inhibition of PI4Kβ by a small molecule inhibitor was also quantitatively assessed, yielding an EC50 value that agrees well with conventional biochemical readout. Taken together, the development of a PI-containing supported membrane platform coupled with surface-sensitive measurement techniques for kinase studies opens the door to exploring the rich biochemistry and pharmacological targeting of membrane-associated phosphoinositides.

Indexed as

1-Phosphatidylinositol 4-KinaseEnzyme InhibitorsLipid BilayersMembrane ProteinsPhosphatidylinositol 4,5-DiphosphatePhosphatidylinositol PhosphatesPhosphotransferases (Alcohol Group Acceptor)Quartz Crystal Microbalance Techniques1-Phosphatidylinositol 4-Kinase1-phosphatidylinositol-4-phosphate 5-kinaseEnzyme InhibitorsLipid BilayersMembrane ProteinsPhosphatidylinositol 4,5-Diphosphatephosphatidylinositol 4-phosphatePhosphatidylinositol PhosphatesPhosphotransferases (Alcohol Group Acceptor)

Identifiers

PMID27118725
PMCPMC5291064
OpenAlexW2344028139

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.