ArticleFASEB journal : official publication of the Federation of American Societies for Experimental Biology2016
Hydroxymethylation is uniquely distributed within term placenta, and is associated with gene expression.
Article in FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Who cites it
28 citing papers in PubMed, 41 citations in OpenAlex.
- Parkinson's disease-associated alterations in DNA methylation and hydroxymethylation in human brain.NPJ Parkinson's disease · 2025Article
- Epigenetic landscape of 5-hydroxymethylcytosine and associations with gene expression in placenta.Epigenetics · 2024Article
- Associations between placental hydroxymethylation and birthweight.Epigenetics reports · 2024Article
- The role of DNA hydroxymethylation and TET enzymes in placental development and pregnancy outcome.Clinical epigenetics · 2023Review
- Prenatal lead (Pb) exposure is associated with differential placental DNA methylation and hydroxymethylation in a human population.Epigenetics · 2022Article
- Placental DNA methylation in pregnancies complicated by maternal diabetes and/or obesity: State of the art and research gaps.Epigenetics · 2022Review
- Navigating the hydroxymethylome: experimental biases and quality control tools for the tandem bisulfite and oxidative bisulfite Illumina microarrays.Epigenomics · 2022Article
- Genome-wide distribution of 5hmC in the dental pulp of mouse molars and incisors.Journal of biochemistry · 2022Article
- DNA 5-hydroxymethylcytosine in pediatric central nervous system tumors may impact tumor classification and is a positive prognostic marker.Clinical epigenetics · 2021Article
- Transcriptomic analysis of primate placentas and novel rhesus trophoblast cell lines informs investigations of human placentation.BMC biology · 2021Article
- Deregulation of imprinted genes expression and epigenetic regulators in placental tissue from intrauterine growth restriction.Journal of assisted reproduction and genetics · 2021Article
- Epigenetic regulation of post-embryonic development.Current opinion in insect science · 2021Review
- Aberrant hydroxymethylation ofEpigenetics · 2020Article
- Supplemental Herbal Choline Increases 5-hmC DNA on Whole Blood from Pregnant Ewes and Offspring.Animals : an open access journal from MDPI · 2020Article
- Genome-wide characterization of cytosine-specific 5-hydroxymethylation in normal breast tissue.Epigenetics · 2020Article
- Characterization of 5-methylcytosine and 5-hydroxymethylcytosine in human placenta cell types across gestation.Epigenetics · 2019Article
- Placental epigenetic clocks: estimating gestational age using placental DNA methylation levels.Aging · 2019Article
- The Role of Epigenetics in Placental Development and the Etiology of Preeclampsia.International journal of molecular sciences · 2019Review
- New guidelines for DNA methylome studies regarding 5-hydroxymethylcytosine for understanding transcriptional regulation.Genome research · 2019Article
- A Novel Application of Mixed Effects Models for Reconciling Base-Pair Resolution 5-Methylcytosine and 5-Hydroxymethylcytosine Data in Neuroepigenetics.Frontiers in genetics · 2019Article
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
Abstract
The conversion of cytosine to 5-methylcystosine (5mC) is an important regulator of gene expression. 5mC may be enzymatically converted to 5-hydroxymethylcytosine (5hmC), with a potentially distinct regulatory function. We sought to investigate these cytosine modifications and their effect on gene expression by parallel processing of genomic DNA using bisulfite and oxidative bisulfite conversion in conjunction with RNA sequencing. Although values of 5hmC across the placental genome were generally low, we identified ∼21,000 loci with consistently elevated levels of 5-hydroxymethycytosine. Absence of 5hmC was observed in CpG islands and, to a greater extent, in non-CpG island-associated regions. 5hmC was enriched within poised enhancers, and depleted within active enhancers, as defined by H3K27ac and H3K4me1 measurements. 5hmC and 5mC were significantly elevated in transcriptionally silent genes when compared with actively transcribed genes. 5hmC was positively associated with transcription in actively transcribed genes only. Our data suggest that dynamic cytosine regulation, associated with transcription, provides the most complete epigenomic landscape of the human placenta, and will be useful for future studies of the placental epigenome.-Green, B. B., Houseman, E. A., Johnson, K. C., Guerin, D. J., Armstrong, D. A., Christensen, B. C., Marsit, C. J. Hydroxymethylation is uniquely distributed within term placenta, and is associated with gene expression.
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