Evidence map›Paper›PMID 27118568›Full record

ArticleInternational urology and nephrology2016

Carbachol-induced signaling through Thr696-phosphorylation of myosin phosphatase-targeting subunit 1 (MYPT1) in rat bladder smooth muscle cells.

Benchun Liu, Yung-Chin Lee, Amjad Alwaal, Guifang Wang, Lia Banie, Ching-Shwun Lin, Guiting Lin, Tom F Lue

Abstract read
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In one paragraph

Article in International urology and nephrology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
0.5field-weighted citation impact, top 29% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed, 7 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 2 institutions in 2 countries.

Benchun LiuKnuppe Molecular Urology Laboratory, Department of Urology, School of Medicine, University of California, 400 Parnassus Ave., Ste A-610, San Francisco, CA, 94143-0738, USA.
Yung-Chin LeeKnuppe Molecular Urology Laboratory, Department of Urology, School of Medicine, University of California, 400 Parnassus Ave., Ste A-610, San Francisco, CA, 94143-0738, USA.
Amjad AlwaalKnuppe Molecular Urology Laboratory, Department of Urology, School of Medicine, University of California, 400 Parnassus Ave., Ste A-610, San Francisco, CA, 94143-0738, USA.
Guifang WangKnuppe Molecular Urology Laboratory, Department of Urology, School of Medicine, University of California, 400 Parnassus Ave., Ste A-610, San Francisco, CA, 94143-0738, USA.
Lia BanieKnuppe Molecular Urology Laboratory, Department of Urology, School of Medicine, University of California, 400 Parnassus Ave., Ste A-610, San Francisco, CA, 94143-0738, USA.
Ching-Shwun LinKnuppe Molecular Urology Laboratory, Department of Urology, School of Medicine, University of California, 400 Parnassus Ave., Ste A-610, San Francisco, CA, 94143-0738, USA.
Guiting LinKnuppe Molecular Urology Laboratory, Department of Urology, School of Medicine, University of California, 400 Parnassus Ave., Ste A-610, San Francisco, CA, 94143-0738, USA.
Tom F LueKnuppe Molecular Urology Laboratory, Department of Urology, School of Medicine, University of California, 400 Parnassus Ave., Ste A-610, San Francisco, CA, 94143-0738, USA. tlue@urology.ucsf.edu.
University of California, San Francisco · USKaohsiung Medical University Chung-Ho Memorial Hospital · TW

Funding

Therapy for Obesity-associated Stress Urinary IncontinenceR01DK105097 · NIDDK · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · PI LUE, TOM F · 2016 to 2019
$2.6M
Therapy for Obesity-associated Stress Urinary IncontinenceR56DK105097 · NIDDK · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · PI LUE, TOM F · 2015 to 2015
$100k
NIDDK NIH HHS R01 DK105097NIDDK NIH HHS R56 DK105097
6 · The paper itself

Abstract

purposeLines of evidence suggest that Rho-associated protein kinase (ROCK)-mediated myosin phosphatase-targeting subunit 1 (MYPT1) phosphorylation plays a central role in smooth muscle contraction. However, the physiological significance of MYPT1 phosphorylation at Thr696 catalyzed by ROCK in bladder smooth muscle remains controversial. We attempt to directly observe the quantitative protein expression of Rho A/ROCK and phosphorylation of MYPT1 at Thr696 after carbachol administration in rat bladder smooth muscle cells (RBMSCs). MATERIALS AND

methodsPrimary cultured smooth muscle cells were obtained from rat bladders. The effects of both concentration and time-course induced by the muscarinic agonist carbachol were investigated by assessing the expression of Rho A/ROCK and MYPT1 phosphorylation at Thr696 using Western blot.

resultsIn the dose-course studies, carbachol showed significant increase in phosphorylation of MYPT1 at Thr696 (p-MYPT1) from concentrations of 15-100 μM based on Western blot results (p < 0.05, ANOVA test). In the time-course studies, treatment of cells with 15 μM of carbachol significantly enhanced the expression of p-MYPT1 from 3 to 15 h (p < 0.05, ANOVA test) and induced the expression of Rho A from 10 to 120 min (p < 0.05, ANOVA test).

conclusionsCarbachol can induce the expression of ROCK pathway, leading to MYPT1 phosphorylation at Thr696 and thereby sustained RBSMCs contraction.

Indexed as

Analysis of VarianceAnimalsBlotting, WesternCarbacholCells, CulturedDose-Response Relationship, DrugMuscle, SmoothMyosin-Light-Chain PhosphatasePhosphorylationRatsRats, Sprague-Dawleyrho-Associated KinasesSensitivity and SpecificitySignal TransductionUrinary BladderCarbacholMyosin-Light-Chain Phosphataserho-Associated KinasesBladder smooth muscle cellCarbacholPhosphorylationRatRho-associated protein kinase (ROCK)

Identifiers

PMID27118568
PMCPMC4955765
OpenAlexW2344365374

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.