ArticleScientific reports2016
A novel serine protease, Sep1, from Bacillus firmus DS-1 has nematicidal activity and degrades multiple intestinal-associated nematode proteins.
Article in Scientific reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
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Who cites it
31 citing papers in PubMed, 101 citations in OpenAlex.
- Biocontrol Potential of Alcaligenes faecalis ZD02 Against the root-knot nematode, Meloidogyne incognita.Current microbiology · 2026Article
- Genomic insights into 'Candidatus Pseudomonas auctus' JDE115, a soybean endophyte with expanded nutrient acquisition and biocontrol-related gene repertoires.BMC genomics · 2026Article
- Metabolomics analysis reveals diverse nematicidal metabolites from spore-forming bacteria.AIMS microbiology · 2026Article
- Isolation and Characterization ofMicroorganisms · 2025Article
- Process optimization and extraction of alkaline protease from halotolerant Streptomyces sp. VITGS3 and its use as a contact lens cleaner.Journal, genetic engineering & biotechnology · 2025Article
- Beyond correlation: Understanding the causal link between microbiome and plant health.Heliyon · 2024Review
- Article
- Employing Bacillus and Pseudomonas for phytonematode management in agricultural crops.World journal of microbiology & biotechnology · 2024Review
- In silico and experimental characterization of a new polyextremophilic subtilisin-like protease from3 Biotech · 2024Article
- Biotechnological Tools to Elucidate the Mechanism of Plant and Nematode Interactions.Plants (Basel, Switzerland) · 2023Review
- Exploring the Biologically Active Metabolites Produced byMetabolites · 2023Article
- Application Potential of Bacterial Volatile Organic Compounds in the Control of Root-Knot Nematodes.Molecules (Basel, Switzerland) · 2022Review
- γ-Polyglutamic Acid Production, Biocontrol, and Stress Tolerance: Multifunction ofInternational journal of environmental research and public health · 2022Article
- Characterization of Nematicidal Activity and Nematode-Toxic Metabolites of a SoilbornePathogens (Basel, Switzerland) · 2022Article
- Nematicidal Effects of Volatile Organic Compounds from Microorganisms and Plants on Plant-Parasitic Nematodes.Microorganisms · 2022Review
- Article
- Root-knot nematodes (Meloidogyne spp.) a threat to agriculture in Mexico: biology, current control strategies, and perspectives.World journal of microbiology & biotechnology · 2022Review
- Identification of the Genes of the Plant PathogenFrontiers in microbiology · 2022Article
- Insights into the phylogeny and transcriptional response of serine proteases in a halotolerant cyanobacteriumPlant signaling & behavior · 2021Article
- Marasmius oreades agglutinin enhances resistance of Arabidopsis against plant-parasitic nematodes and a herbivorous insect.BMC plant biology · 2021Article
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Plant-parasitic nematodes (PPNs) cause serious harm to agricultural production. Bacillus firmus shows excellent control of PPNs and has been produced as a commercial nematicide. However, its nematicidal factors and mechanisms are still unknown. In this study, we showed that B. firmus strain DS-1 has high toxicity against Meloidogyne incognita and soybean cyst nematode. We sequenced the whole genome of DS-1 and identified multiple potential virulence factors. We then focused on a peptidase S8 superfamily protein called Sep1 and demonstrated that it had toxicity against the nematodes Caenorhabditis elegans and M. incognita. The Sep1 protein exhibited serine protease activity and degraded the intestinal tissues of nematodes. Thus, the Sep1 protease of B. firmus is a novel biocontrol factor with activity against a root-knot nematode. We then used C. elegans as a model to elucidate the nematicidal mechanism of Sep1, and the results showed that Sep1 could degrade multiple intestinal and cuticle-associated proteins and destroyed host physical barriers. The knowledge gained in our study will lead to a better understanding of the mechanisms of B. firmus against PPNs and will aid in the development of novel bio-agents with increased efficacy for controlling PPNs.
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