ArticleFitoterapia2016
Countercurrent assisted quantitative recovery of metabolites from plant-associated natural deep eutectic solvents.
Article in Fitoterapia, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
18 citing papers in PubMed, 58 citations in OpenAlex.
- Natural deep eutectic solvents (NaDES): green solvents for pharmaceutical applications and beyond.Green chemistry : an international journal and green chemistry resource : GC · 2025Review
- Exploring Natural Deep Eutectic Solvents (NADES) for Enhanced Essential Oil Extraction: Current Insights and Applications.Molecules (Basel, Switzerland) · 2025Review
- Natural deep eutectic solvents for Ultrasonic-Assisted extraction of nutritious date Sugar: Molecular Screening, Experimental, and prediction.Ultrasonics sonochemistry · 2023Article
- Natural Deep Eutectic Solvents (NADESs) Combined with Sustainable Extraction Techniques: A Review of the Green Chemistry Approach in Food Analysis.Foods (Basel, Switzerland) · 2022Review
- NADES Compounds Identified inPlants (Basel, Switzerland) · 2022Article
- Enhanced Extraction Efficiency of Flavonoids fromMolecules (Basel, Switzerland) · 2022Article
- Natural deep eutectic solvents in phytonutrient extraction and other applications.Frontiers in plant science · 2022Review
- Efficient extraction of bioactive flavonoids fromRSC advances · 2021Article
- Determination of Suitable Macroporous Resins and Desorbents for Carnosol and Carnosic Acid from Deep Eutectic Solvent Sage (Antioxidants (Basel, Switzerland) · 2021Article
- Selective Chlorophyll Removal Method to "Degreen" Botanical Extracts.Journal of natural products · 2020Article
- Green Extraction of Alkaloids and Polyphenols fromPlants (Basel, Switzerland) · 2020Article
- Microwave-assisted deep eutectic solvent extraction of phenolic antioxidants from onion (Journal of food science and technology · 2019Article
- Dynamics of the isoflavone metabolome of traditional preparations of Trifolium pratense L.Journal of ethnopharmacology · 2019Article
- Article
- Article
- Natural Deep Eutectic Solvents: Properties, Applications, and Perspectives.Journal of natural products · 2018Review
- Ionic Liquid-Liquid Chromatography: A New General Purpose Separation Methodology.Topics in current chemistry (Cham) · 2017Review
- Sweet spot matching: A thin-layer chromatography-based countercurrent solvent system selection strategy.Journal of chromatography. A · 2017Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
8 authors at 2 institutions in 1 country.
Funding
Abstract
NAtural Deep Eutectic Solvents (NADES) are chemically simple but physiologically important plant constituents that exhibit unique solubilizing properties of other metabolites, including bioactive constituents. The high polarity of NADES introduces a challenge in the ability of conventional solid-support based chromatography to recover potential bioactive metabolites. This complicates the systematic explanation of the NADES' functions in botanical extracts. The present study utilizes countercurrent separation (CCS) methodology to overcome the recovery challenge. To demonstrate its feasibility, Glucose-Choline chloride-Water (GCWat, 2:5:5, mole/mole) served as a model NADES, and four widely used marker flavonoids with different polarities (rutin, quercetin, kaempferol, and daidzein) were chosen as model target analytes. In order to prepare GCWat with high consistency, a water drying study was performed. The unique capabilities of the recently introduced CherryOne system, offering volumetric phase metering, were used to monitor the CCS operations. The collected fractions were analyzed using UHPLC and NMR/quantitative NMR. CCS was able to recover the analytes from the NADES matrix with quantitative recoveries of 95.7%, 94.6%, 97.0%, and 96.7% for rutin, quercetin, kaempferol, and daidzein respectively. The CCS strategy enables recovery of target metabolites from NADES-containing crude extracts as well as from other chemical mixtures, and moreover offers a means of using NADES as environmentally friendly extraction solvents.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.