ArticleNature communications2016
Optical painting and fluorescence activated sorting of single adherent cells labelled with photoswitchable Pdots.
Article in Nature communications, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
24 citing papers in PubMed.
- Fluorescent Diarylethenes With Polar Groups: Synthesis, Spectra, and Optical Microscopy Applications.Chemistry (Weinheim an der Bergstrasse, Germany) · 2026Article
- Semiconducting polymer dots for multifunctional integrated nanomedicine carriers.Materials today. Bio · 2024Review
- Photoswitching the fluorescence of nanoparticles for advanced optical applications.Chemical science · 2024Review
- Article
- Pooled genetic screens with image-based profiling.Molecular systems biology · 2022Review
- TADF-based NIR-II semiconducting polymer dots forChemical science · 2022Article
- Synergistic Effect of Uroguanylin and DJournal of the American Heart Association · 2022Article
- Gadolinium and Polythiophene Functionalized Polyurea Polymer Dots as Fluoro-Magnetic Nanoprobes.Nanomaterials (Basel, Switzerland) · 2022Article
- Switchable stimulated Raman scattering microscopy with photochromic vibrational probes.Nature communications · 2021Article
- Turn-on mode diarylethenes for bioconjugation and fluorescence microscopy of cellular structures.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- Imaging-based screens of pool-synthesized cell libraries.Nature methods · 2021Review
- Dual-Mode Superresolution Imaging Using Charge Transfer Dynamics in Semiconducting Polymer Dots.Angewandte Chemie (International ed. in English) · 2020Article
- High-throughput, microscope-based sorting to dissect cellular heterogeneity.Molecular systems biology · 2020Article
- Rapid lipolytic oscillations in ex vivo adipose tissue explants revealed through microfluidic droplet sampling at high temporal resolution.Lab on a chip · 2020Article
- Recent advances in microfluidic platforms for single-cell analysis in cancer biology, diagnosis and therapy.Trends in analytical chemistry : TRAC · 2019Article
- Dual-color fluorescent nanoparticles showing perfect color-specific photoswitching for bioimaging and super-resolution microscopy.Nature communications · 2019Article
- Molecular design of near-infrared fluorescent Pdots for tumor targeting: aggregation-induced emissionChemical science · 2019Article
- Thermochemiluminescent semiconducting polymer dots as sensitive nanoprobes for reagentless immunoassay.Nanoscale · 2018Article
- In vivo theranostics with near-infrared-emitting carbon dots-highly efficient photothermal therapy based on passive targeting after intravenous administration.Light, science & applications · 2018Article
- Highly photostable wide-dynamic-range pH sensitive semiconducting polymer dots enabled by dendronizing the near-IR emitters.Chemical science · 2017Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
16 authors.
Funding
Abstract
The efficient selection and isolation of individual cells of interest from a mixed population is desired in many biomedical and clinical applications. Here we show the concept of using photoswitchable semiconducting polymer dots (Pdots) as an optical 'painting' tool, which enables the selection of certain adherent cells based on their fluorescence, and their spatial and morphological features, under a microscope. We first develop a Pdot that can switch between the bright (ON) and dark (OFF) states reversibly with a 150-fold contrast ratio on irradiation with ultraviolet or red light. With a focused 633-nm laser beam that acts as a 'paintbrush' and the photoswitchable Pdots as the 'paint', we select and 'paint' individual Pdot-labelled adherent cells by turning on their fluorescence, then proceed to sort and recover the optically marked cells (with 90% recovery and near 100% purity), followed by genetic analysis.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.