ArticleClinical chemistry2016
A Novel Peptidomic Approach to Strain Typing of Clinical Acinetobacter baumannii Isolates Using Mass Spectrometry.
Article in Clinical chemistry, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. It is linked to trial NCT00077909 (Biomarkers and Protein Mass Expression Profiles in Bronchoalveolar Lavage From Patients With Lung Infiltrates), which is not on this map. Cited by 15 papers.
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Biomarkers and Protein Mass Expression Profiles in Bronchoalveolar Lavage From Patients With Lung Infiltrates
Who cites it
15 citing papers in PubMed.
- Comparison of Raman spectroscopy with mass spectrometry for sequence typing ofMicrobiology spectrum · 2025Article
- Resolving the Temporal Splenic Proteome during Fungal Infection for Discovery of Putative Dual Perspective Biomarker Signatures.Journal of the American Society for Mass Spectrometry · 2023Article
- Parallel Reaction Monitoring Mass Spectrometry for Rapid and Accurate Identification of β-Lactamases Produced byFrontiers in microbiology · 2022Article
- Assay design for unambiguous identification and quantification of circulating pathogen-derived peptide biomarkers.Theranostics · 2022Article
- New approach for the rational selection of markers to identify botulinum toxins.Archives of toxicology · 2021Article
- A Phylogeny-Informed Proteomics Approach for Species Identification within the Burkholderia cepacia Complex.Journal of clinical microbiology · 2020Article
- Rapid and Culture Free Identification ofFrontiers in microbiology · 2020Article
- Fast and Accurate Bacterial Species Identification in Urine Specimens Using LC-MS/MS Mass Spectrometry and Machine Learning.Molecular & cellular proteomics : MCP · 2019Article
- Rapid Identification of New Delhi Metallo-β-Lactamase (NDM) Using Tryptic Peptides and LC-MS/MS.Antimicrobial agents and chemotherapy · 2019Article
- Identification of the OXA-48 Carbapenemase Family by Use of Tryptic Peptides and Liquid Chromatography-Tandem Mass Spectrometry.Journal of clinical microbiology · 2019Article
- Rapid detection of colistin resistance protein MCR-1 by LC-MS/MS.Clinical proteomics · 2019Article
- Deciphering Multifactorial Resistance Phenotypes inMolecular & cellular proteomics : MCP · 2018Article
- Article
- A Genoproteomic Approach to Detect Peptide Markers of Bacterial Respiratory Pathogens.Clinical chemistry · 2017Article
- Peptide Markers for Rapid Detection of KPC Carbapenemase by LC-MS/MS.Scientific reports · 2017Article
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Authors and funding
8 authors.
Funding
Abstract
backgroundAcinetobacter baumannii is a common nosocomial pathogen and strain-typing methods play an important role in hospital outbreak investigations and epidemiologic surveillance. We describe a method for identifying strain-specific peptide markers based on LC-MS/MS profiling of digested peptides. This method classified a test set of A. baumannii isolates collected from a hospital outbreak with discriminatory performance exceeding that of MALDI-TOF mass spectrometry.
methodsFollowing the construction of a species "pan-peptidome" by in silico translation and digestion of whole genome sequences, a hypothetical set of genome-specific peptides for an isolate was constructed from the disjoint set of the pan-peptidome and the isolate's calculated peptidome. The genome-specific peptidome guided selection of highly expressed genome-specific peptides from LC-MS/MS experimental profiles as potential peptide markers. The species specificity of each experimentally identified genome-specific peptide was confirmed through a Unipept lowest common ancestor analysis.
resultsFifteen A. baumannii isolates were analyzed to derive a set of genome- and species-specific peptides that could be used as peptide markers. Identified peptides were cross-checked with protein BLAST against a set of 22 A. baumannii whole genome sequences. A subset of these peptide markers was confirmed to be present in the actual peptide profiles generated by multiple reaction monitoring and targeted LC-MS/MS. The experimentally identified peptides separated these isolates into 6 strains that agreed with multilocus sequence typing analysis performed on the same isolates.
conclusionsThis approach may be generalizable to other bacterial species, and the peptides may be useful for rapid MS strain tracking of isolates with broad application to infectious disease diagnosis.
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