ArticleWorld journal of microbiology & biotechnology2016
A novel process for obtaining pinosylvin using combinatorial bioengineering in Escherichia coli.
Article in World journal of microbiology & biotechnology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 47 citations in OpenAlex.
- Pinosylvin: A Multifunctional Stilbenoid with Antimicrobial, Antioxidant, and Anti-Inflammatory Potential.Current issues in molecular biology · 2025Review
- AFrontiers in plant science · 2024Article
- Sustainable biosynthetic pathways to value-added bioproducts from hydroxycinnamic acids.Applied microbiology and biotechnology · 2023Review
- Efficient biosynthesis of pinosylvin from lignin-derived cinnamic acid by metabolic engineering of Escherichia coli.Biotechnology for biofuels and bioproducts · 2022Article
- Natural Sources and Pharmacological Properties of Pinosylvin.Plants (Basel, Switzerland) · 2022Review
- Synthetic Biology-Driven Microbial Production of Resveratrol: Advances and Perspectives.Frontiers in bioengineering and biotechnology · 2022Review
- Resveratrol Production in Yeast Hosts: Current Status and Perspectives.Biomolecules · 2021Review
- Application of CRISPR/Cas System in the Metabolic Engineering of Small Molecules.Molecular biotechnology · 2021Review
- CRISPR/Cas Technologies and Their Applications inFrontiers in bioengineering and biotechnology · 2021Review
- Application of different types of CRISPR/Cas-based systems in bacteria.Microbial cell factories · 2020Review
- Engineering intracellular malonyl-CoA availability in microbial hosts and its impact on polyketide and fatty acid synthesis.Applied microbiology and biotechnology · 2020Review
- Recent Advances in Metabolically Engineered Microorganisms for the Production of Aromatic Chemicals Derived From Aromatic Amino Acids.Frontiers in bioengineering and biotechnology · 2020Review
- Engineering Plant Secondary Metabolism in Microbial Systems.Plant physiology · 2019Article
- Applications of CRISPR/Cas System to Bacterial Metabolic Engineering.International journal of molecular sciences · 2018Review
- Metabolic Engineering ofFrontiers in microbiology · 2018Article
- Efficient de novo synthesis of resveratrol by metabolically engineered Escherichia coli.Journal of industrial microbiology & biotechnology · 2017Article
- Rational modular design of metabolic network for efficient production of plant polyphenol pinosylvin.Scientific reports · 2017Article
- CRISPR interference (CRISPRi) for gene regulation and succinate production in cyanobacterium S. elongatus PCC 7942.Microbial cell factories · 2016Article
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Pinosylvin as a bioactive stilbene is of great interest for food supplements and pharmaceuticals development. In comparison to conventional extraction of pinosylvin from plant sources, biosynthesis engineering of microbial cell factories is a sustainable and flexible alternative method. Current synthetic strategies often require expensive phenylpropanoic precursor and inducer, which are not available for large-scale fermentation process. In this study, three bioengineering strategies were described to the development of a simple and economical process for pinosylvin biosynthesis in Escherichia coli. Firstly, we evaluated different construct environments to give a highly efficient constitutive system for enzymes of pinosylvin pathway expression: 4-coumarate: coenzyme A ligase (4CL) and stilbene synthase (STS). Secondly, malonyl coenzyme A (malonyl-CoA) is a key precursor of pinosylvin bioproduction and at low level in E. coli cell. Thus clustered regularly interspaced short palindromic repeats interference (CRISPRi) was explored to inactivate malonyl-CoA consumption pathway to increase its availability. The resulting pinosylvin content in engineered E. coli was obtained a 1.9-fold increase depending on the repression of fabD (encoding malonyl-CoA-ACP transacylase) gene. Eventually, a phenylalanine over-producing E. coli consisting phenylalanine ammonia lyase was introduced to produce the precursor of pinosylvin, trans-cinnamic acid, the crude extraction of cultural medium was used as supplementation for pinosylvin bioproduction. Using these combinatorial processes, 47.49 mg/L pinosylvin was produced from glycerol.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.