ArticleInvestigative ophthalmology & visual science2016
Albumen Transport to Bruch's Membrane and RPE by Choriocapillaris Caveolae.
Article in Investigative ophthalmology & visual science, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 36 citations in OpenAlex.
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- A Model of the Mechanisms Underpinning Unconventional Aqueous Humor Outflow.Investigative ophthalmology & visual science · 2025Article
- Comparative analysis of In vivo endothelial cell translatomes across central nervous system vascular beds.Experimental eye research · 2024Article
- Ghost vessels in the eye: Cell free choriocapillaris domains in atrophic age-related macular degeneration.Experimental eye research · 2024Article
- Extent and Topography of Subretinal Drusenoid Deposits Associate With Rod-Mediated Vision in Aging and AMD: ALSTAR2 Baseline.Investigative ophthalmology & visual science · 2024Article
- Choriocapillaris Impairment, Visual Function, and Distance to Fovea in Aging and Age-Related Macular Degeneration: ALSTAR2 Baseline.Investigative ophthalmology & visual science · 2024Article
- APOB100 transgenic mice exemplify how the systemic circulation content may affect the retina without altering retinal cholesterol input.Cellular and molecular life sciences : CMLS · 2024Article
- Delivery Systems in Ocular Retinopathies: The Promising Future of Intravitreal Hydrogels as Sustained-Release Scaffolds.Pharmaceutics · 2023Review
- Caveolar and non-Caveolar Caveolin-1 in ocular homeostasis and disease.Progress in retinal and eye research · 2022Review
- Retinal Pigment Epithelium-Secreted VEGF-A Induces Alpha-2-Macroglobulin Expression in Endothelial Cells.Cells · 2022Article
- Choroidal endothelial and macrophage gene expression in atrophic and neovascular macular degeneration.Human molecular genetics · 2022Article
- Ocular Barriers and Their Influence on Gene Therapy Products Delivery.Pharmaceutics · 2022Review
- Local factor H production by human choroidal endothelial cells mitigates complement deposition: implications for macular degeneration.The Journal of pathology · 2022Article
- Variability in Retinal Neuron Populations and Associated Variations in Mass Transport Systems of the Retina in Health and Aging.Frontiers in aging neuroscience · 2022Review
- Mid-Phase Hyperfluorescent Plaques Seen on Indocyanine Green Angiography in Patients with Central Serous Chorioretinopathy.Journal of clinical medicine · 2021Article
- An optimized confocal intravital microscopy protocol for long-term live imaging of murine F-actin organization during naïve lymphocyte migration.STAR protocols · 2021Article
- Complement System and Potential Therapeutics in Age-Related Macular Degeneration.International journal of molecular sciences · 2021Review
- Bruch's Membrane and the Choroid in Age-Related Macular Degeneration.Advances in experimental medicine and biology · 2021Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
Abstract
purposeThe choriocapillaris (CC), the capillary network of the choroid, is positioned adjacent to Bruch's membrane (BM) and the RPE. The aim of this study was to clarify the mechanism(s) for transport of serum albumen from CC lumen to RPE.
methodsAlexa647 conjugated to BSA (BSA-A647) or PBS was administrated via the femoral vein to young and aged wild-type (WT; C57BL/6J) mice and Caveolin-1 knockout mice (Cav1(-/-)). Mice were perfused with PBS and killed at 30 minutes, 1 hour, and 4 hours after injection. Eyecups were cryopreserved, and cryosections were analyzed on a Zeiss 710 confocal microscope. Bovine serum albumin conjugated to gold nanoparticles (BSA-GNP) was administrated through the left common carotid artery. Mice were perfused with PBS and killed at 30 minutes after injection. Eyecups were embedded after fixation, and 70-nm-thick sections were analyzed on a Hitachi H7600 transmission electron microscope.
resultsIn eyes of WT young mice, BSA-A647 was transported to the RPE at 30 minutes and diffused to the photoreceptor layer by 1 hour. In contrast, most BSA-A647 was found in the CC in Cav1(-/-) eyes. The majority of BSA-GNP found in the CC of young WT mice was on the luminal side in caveolae at 30 minutes after injection. In aged WT mice, BSA-GNPs were found in defective tight junctions between endothelial cells and appeared trapped at the diaphragm of fenestrations.
conclusionsNormally, CC carefully regulates transport system of BSA from lumen to BM by caveolae-mediated transcytosis; however, endothelium cells of aged control WT mice have leaky tight junctions and lacked regulated BSA transport.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.