ArticlePloS one2016
Expression Stabilities of Candidate Reference Genes for RT-qPCR in Chinese Jujube (Ziziphus jujuba Mill.) under a Variety of Conditions.
Article in PloS one, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 39 papers.
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Who cites it
39 citing papers in PubMed, 93 citations in OpenAlex.
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- Identification and validation of reference genes for quantitative real-time PCR in Corydalis saxicola under various abiotic stresses.Scientific reports · 2026Article
- A Novel ZjbZIP33-ZjPRX1 Module Positively Regulates Lignin Formation in the Jujube Fruit Stone.Plant biotechnology journal · 2025Article
- The ZjMYB44-ZjPOD51 module enhances jujube defense response against phytoplasma by upregulating lignin biosynthesis.Horticulture research · 2025Article
- Effects of high-temperature stress on gene expression related to photosynthesis in two jujube (Plant signaling & behavior · 2024Article
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- Identification of ANS gene regulating fruit color in jujube (Ziziphus jujuba Mill. cv. Lingwuchangzao) and analysis of its expression under elevated temperature and drought stress.BMC plant biology · 2024Article
- Mechanism of zju-miR156c-mediated network in regulating witches' broom symptom of Chinese jujube.Molecular plant pathology · 2024Article
- Identification and Analysis ofPlants (Basel, Switzerland) · 2024Article
- Integration analysis of miRNA-mRNA pairs between two contrasting genotypes reveals the molecular mechanism of jujube (Ziziphus jujuba Mill.) response to high-temperature stress.BMC plant biology · 2024Article
- The crotonylated and succinylated proteins of jujube involved in phytoplasma-stress responses.BMC biology · 2024Article
- Optimal Reference Genes for RT-qPCR Experiments in Hippocampus and Cortex of Rats Chronically Exposed to Excessive Fluoride.Biological trace element research · 2024Article
- Anatomical observation and transcriptome analysis of branch-twisted mutations in Chinese jujube.BMC genomics · 2023Article
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- Virus-Induced Gene Silencing (VIGS) in Chinese Jujube.Plants (Basel, Switzerland) · 2023Article
- The genome of Candidatus phytoplasma ziziphi provides insights into their biological characteristics.BMC plant biology · 2023Article
- Screening and Verification of Reference Genes for Analysis of Gene Expression in Garlic (Plants (Basel, Switzerland) · 2023Article
- Genome-wide identification and expression profile analysis of SWEET genes in Chinese jujube.PeerJ · 2023Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Reverse transcription-quantitative real-time polymerase chain reaction (RT-qPCR) is a powerful method for evaluating patterns of gene expression. Jujube whole-genome sequencing has been completed, and analysis of gene function, an important part of any follow-up study, requires the appropriate selection of reference genes. Indeed, suitable reference gene selection for RT-qPCR is critical for accurate normalization of target gene expression. In this study, the software packages geNorm and NormFinder were employed to examine the expression stabilities of nine candidate reference genes under a variety of conditions. Actin-depolymerizing factor 1 (ACT1), Histone-H3 (His3), and Polyadenylate-binding protein-interacting protein (PAIP) were determined to be the most stably expressed genes during five stages of fruit development and ACT1, SiR-Fd, BTF3, and Tubulin alpha chain (TUA) across different tissues/organs. Whereas ACT1, Basic Transcription factor 3 (BTF3), Glyceraldehyde-3-phosphate dehydrogenase (GADPH), and PAIP were the most stable under dark conditions. ACT1, PAIP, BTF3, and Elongation factor 1- gamma (EF1γ) were the most stably expressed genes under phytoplasma infection. Among these genes, SiR-Fd and PAIP are here first reported as stable reference genes. When normalized using these most stable reference genes, the expression patterns of four target genes were found to be in accordance with physiological data, indicating that the reference genes selected in our study are suitable for use in such analyses. This study provides appropriate reference genes and corresponding primers for further RT-qPCR studies in Chinese jujube and emphasizes the importance of validating reference genes for gene expression analysis under variable experimental conditions.
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