Evidence map›Paper›PMID 27116031›Full record

ArticleBiomaterials2016

Metabolic response of lung cancer cells to radiation in a paper-based 3D cell culture system.

Karen A Simon, Bobak Mosadegh, Kyaw Thu Minn, Matthew R Lockett, Marym R Mohammady, Diane M Boucher, Amy B Hall, Shawn M Hillier, Taturo Udagawa, Brenda K Eustace and 1 more

Open access · greenAbstract read
PubMed Publisher
In one paragraph

Article in Biomaterials, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
27citing papers in PubMed, 1 pooled it
6.1field-weighted citation impact, top 3% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

27 citing papers in PubMed, 1 synthesis or guideline pooled it, 76 citations in OpenAlex.

  1. Advances in Engineering Human Tissue Models.Frontiers in bioengineering and biotechnology · 2020
    Pooled it
  2. Review
  3. Article
  4. Cancer cell dynamics on silica fibers.Scientific reports · 2025
    Article
  5. Review
  6. Article
  7. Optically Active, Paper-Based Scaffolds for 3D Cardiac Pacing.ACS applied materials & interfaces · 2024
    Article
  8. Article
  9. Review
  10. Review
  11. Article
  12. Review
  13. Review
  14. Review
  15. Three-dimensional cell culture systems as anWorld journal of stem cells · 2019
    Review
  16. Review
  17. Article
  18. Article
  19. Article
  20. Review
4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

11 authors at 3 institutions in 1 country.

Karen A SimonDepartment of Chemistry and Chemical Biology, Harvard University, 12 Oxford Street, Cambridge, MA 02138, USA.
Bobak MosadeghDepartment of Chemistry and Chemical Biology, Harvard University, 12 Oxford Street, Cambridge, MA 02138, USA; Wyss Institute for Biologically Inspired Engineering, Harvard University, 60 Oxford Street, Cambridge, MA 02138, USA; Dalio Institute of Cardiovascular Imaging, Department of Radiology, Weill Cornell Medicine, 413 E. 69th Street Suite BRB-108, New York, NY 10021, USA.
Kyaw Thu MinnDepartment of Chemistry and Chemical Biology, Harvard University, 12 Oxford Street, Cambridge, MA 02138, USA.
Matthew R LockettDepartment of Chemistry and Chemical Biology, Harvard University, 12 Oxford Street, Cambridge, MA 02138, USA; Department of Chemistry, University of North Carolina at Chapel Hill, 125 South Road, Chapel Hill, NC 27599, USA.
Marym R MohammadyDepartment of Chemistry and Chemical Biology, Harvard University, 12 Oxford Street, Cambridge, MA 02138, USA.
Diane M BoucherVertex Pharmaceuticals Incorporated, 50 Northern Blvd., Boston, MA 02210, USA.
Amy B HallVertex Pharmaceuticals Incorporated, 50 Northern Blvd., Boston, MA 02210, USA.
Shawn M HillierVertex Pharmaceuticals Incorporated, 50 Northern Blvd., Boston, MA 02210, USA.
Taturo UdagawaVertex Pharmaceuticals Incorporated, 50 Northern Blvd., Boston, MA 02210, USA.
Brenda K EustaceVertex Pharmaceuticals Incorporated, 50 Northern Blvd., Boston, MA 02210, USA. Electronic address: brenda_eustace@vrtx.com.
George M WhitesidesDepartment of Chemistry and Chemical Biology, Harvard University, 12 Oxford Street, Cambridge, MA 02138, USA; Wyss Institute for Biologically Inspired Engineering, Harvard University, 60 Oxford Street, Cambridge, MA 02138, USA. Electronic address: gwhitesides@gmwgroup.harvard.edu.
Harvard University · USVertex Pharmaceuticals (United States) · USUniversity of North Carolina at Chapel Hill · US

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

This work demonstrates the application of a 3D culture system-Cells-in-Gels-in-Paper (CiGiP)-in evaluating the metabolic response of lung cancer cells to ionizing radiation. The 3D tissue-like construct-prepared by stacking multiple sheets of paper containing cell-embedded hydrogels-generates a gradient of oxygen and nutrients that decreases monotonically in the stack. Separating the layers of the stack after exposure enabled analysis of the cellular response to radiation as a function of oxygen and nutrient availability; this availability is dictated by the distance between the cells and the source of oxygenated medium. As the distance between the cells and source of oxygenated media increased, cells show increased levels of hypoxia-inducible factor 1-alpha, decreased proliferation, and reduced sensitivity to ionizing radiation. Each of these cellular responses are characteristic of cancer cells observed in solid tumors. With this setup we were able to differentiate three isogenic variants of A549 cells based on their metabolic radiosensitivity; these three variants have known differences in their metastatic behavior in vivo. This system can, therefore, capture some aspects of radiosensitivity of populations of cancer cells related to mass-transport phenomenon, carry out systematic studies of radiation response in vitro that decouple effects from migration and proliferation of cells, and regulate the exposure of oxygen to subpopulations of cells in a tissue-like construct either before or after irradiation.

Indexed as

A549 CellsCell Culture TechniquesCell ProliferationCell SurvivalHumansHydrogelsHypoxia-Inducible Factor 1, alpha SubunitLung NeoplasmsOxygenPaperRadiation ToleranceTumor HypoxiaHydrogelsHypoxia-Inducible Factor 1, alpha SubunitOxygen3D cell cultureOxygen gradientsRadiation responseRadioresistanceTumor hypoxia

Identifiers

PMID27116031
OpenAlexW2289291437

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.