Evidence map›Paper›PMID 27114694›Full record

ArticlePharmacognosy research2016

Extracts from Flammulina velutipes Inhibit the Adhesion of Pathogenic Fungi to Epithelial Cells.

Svetlana Kashina, Lérida Liss Flores Villavicencio, Marco Balleza, Gloria Barbosa Sabanero, Víctor Tsutsumi, Myrna Sabanero López

Open access · hybridAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Pharmacognosy research, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
0.4field-weighted citation impact, top 36% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed, 4 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 1 institution in 1 country.

Svetlana KashinaDepartment of Biology, Division of Natural and Exact Sciences, Campus Guanajuato, University of Guanajuato, Noria Alta S/N, Col. Noria Alta, Guanajuato, Guanajuato 36050, México City, México.
Lérida Liss Flores VillavicencioDepartment of Biology, Division of Natural and Exact Sciences, Campus Guanajuato, University of Guanajuato, Noria Alta S/N, Col. Noria Alta, Guanajuato, Guanajuato 36050, México City, México.
Marco BallezaDepartment of Science and Engineering, Campus León, University of Guanajuato, Loma del Bosque 103 Col, Lomas del Campestre, León, Guanajuato 37150, México City, México.
Gloria Barbosa SabaneroDepartment of Medical Sciences, Division of Health Sciences, Campus León, University of Guanajuato, 20 de Enero 292, Col. Obregón, León, Guanajuato 37320, México City, México.
Víctor TsutsumiDepartment of Infectomics and Molecular Pathogenesis, Center for Research and Advanced Studies, National Polytechnic Institute (CINVESTAV-IPN), Av. Instituto Politécnico Nacional 2508, San Pedro Zacatenco, Gustavo A, Madero, 07360, México City, México.
Myrna Sabanero LópezDepartment of Biology, Division of Natural and Exact Sciences, Campus Guanajuato, University of Guanajuato, Noria Alta S/N, Col. Noria Alta, Guanajuato, Guanajuato 36050, México City, México.
Universidad de Guanajuato · MX

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundRecently, extracts from natural sources have been tested for their antifungal properties. In this aspect, Flammulina velutipes extracts possess a significant amount of branch-chained carbohydrates with mannose moieties that, hypothetically, can reduce the adhesion.

objectiveIn this study, we assessed the capacity of extracts from F. velutipes (wild-type AQF-1 and ATCC 34574 as the reference strain) to inhibit the adhesion of S. schenkii and C. albicans to epithelial cells. MATERIALS AND

methodsThe aqueous extracts from F. velutipes strains were obtained by sonication, total carbohydrate and protein was analyzed by Dubois and Lowry methods respectively. Effect of the extracts (50, 100 and 150 μg/mL) on the fungi adhesion to host cells was evaluated after 1 h interaction, and the percentage of inhibition of adhesion was measured. After of interaction the cytoskeleton from cell was analyzed with phalloidin-FITC.

resultsThe extract from strain AQF-1 (50, 100 and 150 μg/mL) inhibited the adhesion of: S. schenkii in a dose-dependent manner (4.9, 7.5 and 12.7%, respectively) and C. albicans in a dose-independent manner (5.2%). The percentage of inhibition by extracts from the strain ATCC34574 at the same concentrations, shown that are dose independent for both fungi: 3.9% for S. schenkii and 2.6% for C. albicans.

conclusionThe extracts from F. velutipes inhibit the adhesion of pathogenic fungi to host cells. The mechanism molecular is unknown; however, is probably an interaction between the polysaccharides from extracts with the fungi receptors. This aspect is currently analyzed. SUMMARY: The yields of mycelium from two strains of F. velutipes and the extract from it were similar.Extracts from both strains have inhibited adhesion of S. schenkii and C. albicans to epithelial cells in vitro, but the extract from strain AQF-1 was more effective.The extracts have not prevented damage to epithelial cells caused by pathogenic fungi. Abbreviation Used: YPG: Yeast peptone glucose, DMEM: Dulbecco's Modified Eagle's medium, FITC: Fluorescein isothiocyanate.

Indexed as

Adhesionbasidiomycets extractsepithelial cellsFlammulina velutipesfungi

Identifiers

PMID27114694
PMCPMC4821109
OpenAlexW2297019974

What OpenQuestion holds

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LicenceCC BY-NC-SA
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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.