ArticleProceedings of the National Academy of Sciences of the United States of America2016
The Entner-Doudoroff pathway is an overlooked glycolytic route in cyanobacteria and plants.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 94 papers.
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94 citing papers in PubMed, 200 citations in OpenAlex.
- Article
- The (Glg)ABCs of cyanobacteria: modelling of glycogen synthesis and functional divergence of glycogen synthases in Synechocystis sp. PCC 6803.FEBS letters · 2026Article
- The return of metabolism: biochemistry and physiology of glycolysis.Biological reviews of the Cambridge Philosophical Society · 2026Review
- Enhanced fumarate production using high-density cultivation of Synechocystis sp. PCC 6803.Microbial cell factories · 2026Article
- Genomes of NBMC genomics · 2026Article
- Controlled Carbon Loss: Threshold-Dependent Overflow Metabolism inMicroorganisms · 2025Article
- Fructose-1,6-bisphosphatase is involved in heterotrophic growth and glycogen metabolism in cyanobacteria.Journal of experimental botany · 2025Article
- Development and multi-center validation of machine learning models based on targeted metabolomics for rheumatoid arthritis.Journal of translational medicine · 2025Article
- Microbial synthesis of branched-chain β,γ-diols from amino acid metabolism.Nature communications · 2025Article
- Permeability selection of biologically relevant membranes matches the stereochemistry of life on Earth.PLoS biology · 2025Article
- Chimeric origins and dynamic evolution of central carbon metabolism in eukaryotes.Nature ecology & evolution · 2025Article
- Gut Microbiome Alterations, Mental Health, and Alcohol Consumption: Investigating the Gut-Brain Axis in Firefighters.Microorganisms · 2025Article
- Dynamic photosynthetic labeling and carbon-positional mass spectrometry monitor in vivo RUBISCO carbon assimilation rates.Plant physiology · 2025Article
- The early evolution of the glycolytic pathway from autotrophic origins to glycogen and back.FEMS microbiology reviews · 2025Review
- Advances in seed hypoxia research.Plant physiology · 2024Article
- Phosphoketolase and KDPG aldolase metabolisms modulate photosynthetic carbon yield in cyanobacteria.The Plant cell · 2024Article
- Structure function analysis of ADP-dependent cyanobacterial phosphofructokinase reveals new phylogenetic grouping in the PFK-A family.The Journal of biological chemistry · 2024Article
- A quantitative description of light-limited cyanobacterial growth using flux balance analysis.PLoS computational biology · 2024Article
- Interplay between photosynthetic electron flux and organic carbon sinks in sucrose-excreting Synechocystis sp. PCC 6803 revealed by omics approaches.Microbial cell factories · 2024Article
- Double blocking of carbon metabolism causes a large increase of Calvin-Benson cycle compounds in cyanobacteria.Plant physiology · 2024Article
34 more citing papers are in PubMed but not listed here.
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11 authors at 3 institutions in 1 country.
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No grant is acknowledged in the PubMed record.
Abstract
Glucose degradation pathways are central for energy and carbon metabolism throughout all domains of life. They provide ATP, NAD(P)H, and biosynthetic precursors for amino acids, nucleotides, and fatty acids. It is general knowledge that cyanobacteria and plants oxidize carbohydrates via glycolysis [the Embden-Meyerhof-Parnas (EMP) pathway] and the oxidative pentose phosphate (OPP) pathway. However, we found that both possess a third, previously overlooked pathway of glucose breakdown: the Entner-Doudoroff (ED) pathway. Its key enzyme, 2-keto-3-deoxygluconate-6-phosphate (KDPG) aldolase, is widespread in cyanobacteria, moss, fern, algae, and plants and is even more common among cyanobacteria than phosphofructokinase (PFK), the key enzyme of the EMP pathway. Active KDPG aldolases from the cyanobacterium Synechocystis and the plant barley (Hordeum vulgare) were biochemically characterized in vitro. KDPG, a metabolite unique to the ED pathway, was detected in both in vivo, indicating an active ED pathway. Phylogenetic analyses revealed that photosynthetic eukaryotes acquired KDPG aldolase from the cyanobacterial ancestors of plastids via endosymbiotic gene transfer. Several Synechocystis mutants in which key enzymes of all three glucose degradation pathways were knocked out indicate that the ED pathway is physiologically significant, especially under mixotrophic conditions (light and glucose) and under autotrophic conditions in a day/night cycle, which is probably the most common condition encountered in nature. The ED pathway has lower protein costs and ATP yields than the EMP pathway, in line with the observation that oxygenic photosynthesizers are nutrient-limited, rather than ATP-limited. Furthermore, the ED pathway does not generate futile cycles in organisms that fix CO2 via the Calvin-Benson cycle.
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