ArticleProceedings of the National Academy of Sciences of the United States of America2016
Tracking solvent and protein movement during CO2 release in carbonic anhydrase II crystals.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 39 citations in OpenAlex.
- Conformational flexibility of His200 enables catalytic activity in the T200H mutant of carbonic anhydrase II.Molecules and cells · 2025Article
- Fast product release requires active-site water dynamics in carbonic anhydrase.Nature communications · 2025Article
- Hidden route of protein damage through oxygen-confined photooxidation.Nature communications · 2024Article
- Carbonic Anhydrase Robustness for Use in Nanoscale COACS omega · 2023Article
- Switchable aqueous catalytic systems for organic transformations.Communications chemistry · 2022Review
- Scaffolding protein functional sites using deep learning.Science (New York, N.Y.) · 2022Article
- Characterization of a novel type of carbonic anhydrase that acts without metal cofactors.BMC biology · 2021Article
- The Active Site of a Prototypical "Rigid" Drug Target is Marked by Extensive Conformational Dynamics.Angewandte Chemie (International ed. in English) · 2020Article
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- Elucidating the role of metal ions in carbonic anhydrase catalysis.Nature communications · 2020Article
- Catalytically Active Peptide-Gold Nanoparticle Conjugates: Prospecting for Artificial Enzymes.Angewandte Chemie (International ed. in English) · 2020Review
- Article
- Redox-coupled proton pumping drives carbon concentration in the photosynthetic complex I.Nature communications · 2020Article
- Structural comparison of protiated, H/D-exchanged and deuterated human carbonic anhydrase IX.Acta crystallographica. Section D, Structural biology · 2019Article
- Activation of β- and γ-carbonic anhydrases from pathogenic bacteria with tripeptides.Journal of enzyme inhibition and medicinal chemistry · 2018Article
- Carbonic anhydrase II microcrystals suitable for XFEL studies.Acta crystallographica. Section F, Structural biology communications · 2018Article
- The Crystal Structure of a hCA VII Variant Provides Insights into the Molecular Determinants Responsible for Its Catalytic Behavior.International journal of molecular sciences · 2018Article
- Carbonic anhydrase under pressure.IUCrJ · 2018Article
- Active-site solvent replenishment observed during human carbonic anhydrase II catalysis.IUCrJ · 2018Article
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Authors and funding
6 authors at 4 institutions in 2 countries.
Funding
Abstract
Carbonic anhydrases are mostly zinc metalloenzymes that catalyze the reversible hydration/dehydration of CO2/HCO3 (-) Previously, the X-ray crystal structures of CO2-bound holo (zinc-bound) and apo (zinc-free) human carbonic anhydrase IIs (hCA IIs) were captured at high resolution. Here, we present sequential timeframe structures of holo- [T = 0 s (CO2-bound), 50 s, 3 min, 10 min, 25 min, and 1 h] and apo-hCA IIs [T = 0 s, 50 s, 3 min, and 10 min] during the "slow" release of CO2 Two active site waters, WDW (deep water) and WDW' (this study), replace the vacated space created on CO2 release, and another water, WI (intermediate water), is seen to translocate to the proton wire position W1. In addition, on the rim of the active site pocket, a water W2' (this study), in close proximity to residue His64 and W2, gradually exits the active site, whereas His64 concurrently rotates from pointing away ("out") to pointing toward ("in") active site rotameric conformation. This study provides for the first time, to our knowledge, structural "snapshots" of hCA II intermediate states during the formation of the His64-mediated proton wire that is induced as CO2 is released. Comparison of the holo- and apo-hCA II structures shows that the solvent network rearrangements require the presence of the zinc ion.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.