ArticleThe Journal of cell biology2016
Remodeling the zonula adherens in response to tension and the role of afadin in this response.
Article in The Journal of cell biology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 130 papers.
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Who cites it
130 citing papers in PubMed, 212 citations in OpenAlex.
- Zyxin and LPP differ in their sensing of strained actin filaments at tricellular junctions.bioRxiv : the preprint server for biology · 2026Article
- Tight junction structure, assembly and (dys)function.Nature reviews. Molecular cell biology · 2026Review
- SGEF coordinates epithelial morphogenesis by regulating junction stability, collective migration, and extracellular matrix remodeling.bioRxiv : the preprint server for biology · 2026Article
- ZO-1 shuttles between apical junctional complexes and podosomes by riding ERK activation waves.Nature communications · 2026Article
- Two δ-catenins, plakophilin 4 and p120, promote formation of distinct types of adherens junctions.The Journal of cell biology · 2026Article
- Genetically engineered ESC-derived embryos reveal Vinculin-dependent force responses required for mammalian neural tube closure.bioRxiv : the preprint server for biology · 2025Article
- A key role of Canoe's intrinsically disordered region in linking cell junctions to the cytoskeleton.The Journal of cell biology · 2025Article
- Regulation of epithelial tissue homeostasis by active transepithelial transport.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- PAK4 promotes vertex remodeling to maintain epithelial integrity and barrier function.bioRxiv : the preprint server for biology · 2025Article
- Mushroom bodies tiny regulates Sidekick localization to tricellular adherens junctions.Development (Cambridge, England) · 2025Article
- Roles of protein-protein interactions and monolayer mechanics in tricellulin localization to tricellular tight junctions.Biology open · 2025Article
- α-catenin phosphorylation is actomyosin-sensitive and required for epithelial barrier functions through Afadin.bioRxiv : the preprint server for biology · 2025Article
- Combined Pharmacological Conditioning of Endothelial Cells for Improved Vascular Graft Endothelialization.International journal of molecular sciences · 2025Article
- Mechanical cues polarize ADIP protein complexes to control vertebrate morphogenesis and wound healing.Current biology : CB · 2025Article
- F-actin dynamics regulates collective cell migration by modulating cell shape and stress correlation.Biophysical journal · 2025Article
- Afadin loss induces breast cancer metastasis through destabilisation of E-cadherin to F-actin linkage.The Journal of pathology · 2025Article
- Vertex models capturing subcellular scales in epithelial tissues.PLoS computational biology · 2025Review
- Proximity proteomics provides a new resource for exploring the function of Afadin and the complexity of cell-cell adherens junctions.Biology open · 2025Article
- Afadin mediates cadherin-catenin complex clustering on F-actin linked to cooperative binding and filament curvature.Science advances · 2025Article
- Inferring active and passive mechanical drivers of epithelial convergent extension.bioRxiv : the preprint server for biology · 2025Article
70 more citing papers are in PubMed but not listed here.
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Authors and funding
9 authors at 4 institutions in 4 countries.
Funding
Abstract
Morphogenesis requires dynamic coordination between cell-cell adhesion and the cytoskeleton to allow cells to change shape and move without losing tissue integrity. We used genetic tools and superresolution microscopy in a simple model epithelial cell line to define how the molecular architecture of cell-cell zonula adherens (ZA) is modified in response to elevated contractility, and how these cells maintain tissue integrity. We previously found that depleting zonula occludens 1 (ZO-1) family proteins in MDCK cells induces a highly organized contractile actomyosin array at the ZA. We find that ZO knockdown elevates contractility via a Shroom3/Rho-associated, coiled-coil containing protein kinase (ROCK) pathway. Our data suggest that each bicellular border is an independent contractile unit, with actin cables anchored end-on to cadherin complexes at tricellular junctions. Cells respond to elevated contractility by increasing junctional afadin. Although ZO/afadin knockdown did not prevent contractile array assembly, it dramatically altered cell shape and barrier function in response to elevated contractility. We propose that afadin acts as a robust protein scaffold that maintains ZA architecture at tricellular junctions.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.