ArticleJournal of biomaterials applications2016
Optimization of alginate purification using polyvinylidene difluoride membrane filtration: Effects on immunogenicity and biocompatibility of three-dimensional alginate scaffolds.
Article in Journal of biomaterials applications, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 44 citations in OpenAlex.
- SA/HA double-network hydrogel combined with PVDF electret membrane enhances osteogenic differentiation of DPSCs and promotes mandibular bone defect repair.Regenerative therapy · 2026Article
- Advances in alginate biosynthesis: regulation and production inFrontiers in bioengineering and biotechnology · 2025Review
- Tunable Oxidized-Chitin Hydrogels with Customizable Mechanical Properties by Metal or Hydrogen Ion Exposure.Marine drugs · 2024Article
- A Case for Material Stiffness as a Design Parameter in Encapsulated Islet Transplantation.Tissue engineering. Part B, Reviews · 2023Review
- A Quick and Efficient Method for the Generation of Immunomodulatory Mesenchymal Stromal Cell from Human Induced Pluripotent Stem Cell.Tissue engineering. Part A · 2022Article
- Layer-by-Layer Cell Encapsulation for Drug Delivery: The History, Technique Basis, and Applications.Pharmaceutics · 2022Review
- Effects of Three-Dimensional Sodium Alginate Scaffold on Maturation and Developmental Gene Expressions in Fresh and Vitrified Preantral Follicles of Mice.International journal of fertility & sterility · 2021Article
- Encapsulated Mesenchymal Stromal Cell Microbeads Promote Endogenous Regeneration of Osteoarthritic Cartilage Ex Vivo.Advanced healthcare materials · 2021Article
- Modulating Alginate Hydrogels for Improved Biological Performance as Cellular 3D Microenvironments.Frontiers in bioengineering and biotechnology · 2020Review
- Polymeric Approaches to Reduce Tissue Responses Against Devices Applied for Islet-Cell Encapsulation.Frontiers in bioengineering and biotechnology · 2019Review
- RGDfK-Peptide Modified Alginate Scaffold for Cell Transplantation and Cardiac Neovascularization.Tissue engineering. Part A · 2018Article
- Improved BALB/c mice granulosa cell functions using purified alginate scaffold.Iranian journal of veterinary research · 2018Article
- Alginate-encapsulated brain-derived neurotrophic factor-overexpressing mesenchymal stem cells are a promising drug delivery system for protection of auditory neurons.Journal of tissue engineeringArticle
Corrections and comments
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Authors and funding
8 authors at 5 institutions in 2 countries.
Funding
Abstract
Sodium alginate is an effective biomaterial for tissue engineering applications. Non-purified alginate is contaminated with protein, lipopolysaccharide, DNA, and RNA, which could elicit adverse immunological reactions. We developed a purification protocol to generate biocompatible alginate based on (a) activated charcoal treatment, (b) use of hydrophobic membrane filtration (we used hydrophobic polyvinylidene difluoride membranes to remove organic contaminants), (c) dialysis, and finally (d) ethanol precipitation. Using this approach, we could omit pre-treatment with chloroform and significantly reduce the quantities of reagents used. Purification resulted in reduction of residual protein by 70% down to 0.315 mg/g, DNA by 62% down to 1.28 µg/g, and RNA by 61% down to less than 10 µg/g, respectively. Lipopolysaccharide levels were reduced by >90% to less than 125 EU/g. Purified alginate did not induce splenocyte proliferation in vitro. Three-dimensional scaffolds generated from purified alginate did not elicit a significant foreign body reaction, fibrotic overgrowth, or macrophage infiltration 4 weeks after implantation. This study describes a simplified and economical alginate purification method that results in alginate purity, which meets clinically useful criteria.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.