Evidence map›Paper›PMID 27114041›Full record

ArticleAnalytical biochemistry2016

Structure determination of lipopeptides from Mycobacterium avium subspecies paratuberculosis and identification of antigenic lipopeptide probes.

Katsuhiko Mitachi, Lekh Nath Sharma Gautam, Jeffrey H Rice, Keiko Eda, Ashutosh Wadhwa, Eiichi Momotani, Joseph P Hlopak, Shigetoshi Eda, Michio Kurosu

Abstract read
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In one paragraph

Article in Analytical biochemistry, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.2field-weighted citation impact, top 38% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 3 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 4 institutions in 2 countries.

Katsuhiko MitachiDepartment of Pharmaceutical Sciences, College of Pharmacy, University of Tennessee Health Science Center, Memphis, TN, 38163, USA.
Lekh Nath Sharma GautamDepartment of Pharmaceutical Sciences, College of Pharmacy, University of Tennessee Health Science Center, Memphis, TN, 38163, USA.
Jeffrey H RiceCenter for Wildlife Health, Department of Forestry, Wildlife, and Fisheries, University of Tennessee, Knoxville, TN, 37996, USA.
Keiko EdaCenter for Wildlife Health, Department of Forestry, Wildlife, and Fisheries, University of Tennessee, Knoxville, TN, 37996, USA.
Ashutosh WadhwaCenter for Wildlife Health, Department of Forestry, Wildlife, and Fisheries, University of Tennessee, Knoxville, TN, 37996, USA.
Eiichi MomotaniDepartment of Human Care, Tohto College of Health Sciences, Nishi Fukaya, Saitama, 366-0052, Japan.
Joseph P HlopakCenter for Wildlife Health, Department of Forestry, Wildlife, and Fisheries, University of Tennessee, Knoxville, TN, 37996, USA.
Shigetoshi EdaCenter for Wildlife Health, Department of Forestry, Wildlife, and Fisheries, University of Tennessee, Knoxville, TN, 37996, USA.
Michio KurosuDepartment of Pharmaceutical Sciences, College of Pharmacy, University of Tennessee Health Science Center, Memphis, TN, 38163, USA. Electronic address: mkurosu@uthsc.edu.
University of Tennessee at Knoxville · USUniversity of Tennessee Health Science Center · USLouisiana Department of Wildlife and Fisheries · USTohto College of Health Sciences · JP

Funding

Targeting MraY: Synthesis and Validation of MraY InhibitorsR01AI084411 · NIAID · UNIVERSITY OF TENNESSEE HEALTH SCI CTR · PI KUROSU, MICHIO · 2010 to 2013
$1.5M
Expansion of spectrum of activity of pleuromutilin for MDR Gram-negative bacteriaR21AI119796 · NIAID · UNIVERSITY OF TENNESSEE HEALTH SCI CTR · PI KUROSU, MICHIO · 2015 to 2016
$431k
NIAID NIH HHS R01 AI084411NIAID NIH HHS R21 AI119796
6 · The paper itself

Abstract

Mycobacterium avium subspecies paratuberculosis (MAP) causes chronic illnesses mostly in ruminants. MAP infection of intestinal tissue triggers a fatal inflammatory disorder, Johne's disease (paratuberculosis). Development of fast and reliable diagnostic methods for Johne's disease in clinically suspected ruminants requires the discovery of MAP-specific antigens that induce immune responses. Despite a longtime interest in finding such antigens that can detect serum antibody responses with high sensitivity, the antigens currently used for a diagnosis of the MAP infections are the crude extracts from the whole cell. We performed the serum antibody response assay-guided purification of the ethanol extract from MAP isolated from an infected cow. With the results of extensive fractionations and in vitro assays, we identified that arachidyl-d-Phe-N-Me-l-Val-l-Ile-l-Phe-l-Ala-OH (named lipopeptide IIß, 3) exhibited the highest antibody binding activity in serum of a MAP-infected cattle compared with the other lipopeptides isolated from MAP. The absolute chemistry of 3 was determined unequivocally via our high-performance liquid chromatography (HPLC)-amino acid databases. α-Amino lipopeptide IIß and its fluorescent probes were synthesized and evaluated in serum antibody binding activity assays. Lipopeptide IIß-(2S)-NH2 (9) and its dansyl and fluorescein isothiocyanate (FITC) probes (10 and 11) exhibited antibody-mediated binding activity; thus, such MAP-specific lipopeptide probes can be potential biomarkers for the development of rapid and accurate diagnosis of Johne's disease.

Indexed as

AnimalsAntibodies, BacterialAntigens, BacterialCattleFluorescent DyesLipopeptidesMycobacterium avium subsp. paratuberculosisProtein ConformationAntibodies, BacterialAntigens, BacterialFluorescent DyesLipopeptidesJohne's diseaseLipopeptideLipopeptide probesMycobacterium avium subspecies paratuberculosisNew chiral reprivatizing agents

Identifiers

PMID27114041
PMCPMC4899129
OpenAlexW2338239202

What OpenQuestion holds

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Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.