Evidence map›Paper›PMID 27113852›Full record

ArticleThe Journal of endocrinology2016

Progestin increases the expression of gonadotropins in pituitaries of male zebrafish.

Cuili Wang, Dongteng Liu, Weiting Chen, Wei Ge, Wanshu Hong, Yong Zhu, Shi X Chen

Open access · bronzeAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in The Journal of endocrinology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed
3.2field-weighted citation impact, top 9% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

6 citing papers in PubMed, 19 citations in OpenAlex.

  1. Article
  2. Regulation of spermatogenesis and reproductive capacity by Igf3 in tilapia.Cellular and molecular life sciences : CMLS · 2020
    Article
  3. Article
  4. Review
  5. Article
  6. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 4 institutions in 3 countries.

Cuili WangState Key Laboratory of Marine Environmental ScienceCollege of Ocean and Earth Sciences, Xiamen University, Xiamen, China Fujian Collaborative Innovation Center for Exploitation and Utilization of Marine Biological ResourcesXiamen, China.
Dongteng LiuState Key Laboratory of Marine Environmental ScienceCollege of Ocean and Earth Sciences, Xiamen University, Xiamen, China Fujian Collaborative Innovation Center for Exploitation and Utilization of Marine Biological ResourcesXiamen, China.
Weiting ChenCentre of ReproductionDevelopment and Aging (CRDA), Faculty of Health Sciences, University of Macau, Taipa, Macau, China.
Wei GeCentre of ReproductionDevelopment and Aging (CRDA), Faculty of Health Sciences, University of Macau, Taipa, Macau, China.
Wanshu HongState Key Laboratory of Marine Environmental ScienceCollege of Ocean and Earth Sciences, Xiamen University, Xiamen, China Fujian Collaborative Innovation Center for Exploitation and Utilization of Marine Biological ResourcesXiamen, China.
Yong ZhuState Key Laboratory of Marine Environmental ScienceCollege of Ocean and Earth Sciences, Xiamen University, Xiamen, China Department of BiologyEast Carolina University, Greenville, North Carolina, USA.
Shi X ChenState Key Laboratory of Marine Environmental ScienceCollege of Ocean and Earth Sciences, Xiamen University, Xiamen, China Fujian Collaborative Innovation Center for Exploitation and Utilization of Marine Biological ResourcesXiamen, China State-Province Joint Engineering Laboratory of Marine Bioproducts and TechnologyXiamen University, Xiamen, China chenshixi@xmu.edu.cn.
Collaborative Innovation Center of Chemistry for Energy Materials · CNUniversity of Macau · MOXiamen University · CNFujian Fisheries Research Institute · CN

Funding

To characterize nongenomic progestin receptors via knockouts in zebrafishR15GM100461 · NIGMS · EAST CAROLINA UNIVERSITY · PI ZHU, YONG · 2013 to 2017
$757k
NIGMS NIH HHS R15 GM100461
6 · The paper itself

Abstract

Our previous study showed that the in vivo positive effects of 17α,20β-dihydroxy-4-pregnen-3-one (DHP), the major progestin in zebrafish, on early spermatogenesis was much stronger than the ex vivo ones, which may suggest an effect of DHP on the expression of gonadotropins. In our present study, we first observed that fshb and lhb mRNA levels in the pituitary of male adult zebrafish were greatly inhibited by 3 weeks exposure to 10nM estradiol (E2). However, an additional 24h 100nM DHP exposure not only reversed the E2-induced inhibition, but also significantly increased the expression of fshb and lhb mRNA. These stimulatory effects were also observed in male adult fish without E2 pretreatment, and a time course experiment showed that it took 24h for fshb and 12h for lhb to respond significantly. Because these stimulatory activities were partially antagonized by a nuclear progesterone receptor (Pgr) antagonist mifepristone, we generated a Pgr-knockout (pgr(-/-)) model using the TALEN technique. With and without DHP in vivo treatment, fshb and lhb mRNA levels of pgr(-/-) were significantly lower than those of pgr(+/+) Furthermore, ex vivo treatment of pituitary fragments of pgr(-/-) with DHP stimulated lhb, but not fshb mRNA expression. Results from double-colored fluorescent in situ hybridization showed that pgr mRNA was expressed only in fshb-expressing cells. Taken together, our results indicated that DHP participated in the regulation of neuroendocrine control of reproduction in male zebrafish, and exerted a Pgr-mediated direct stimulatory effect on fshb mRNA at pituitary level.

Indexed as

AnimalsAnimals, Genetically ModifiedEstradiolFollicle Stimulating Hormone, beta SubunitGene ExpressionHormone AntagonistsLuteinizing Hormone, beta SubunitMaleMifepristonePituitary GlandProgestinsReceptors, ProgesteroneZebrafishEstradiolFollicle Stimulating Hormone, beta SubunitHormone AntagonistsLuteinizing Hormone, beta SubunitMifepristoneProgestinsReceptors, Progesterone17α,20β-dihydroxy-4-pregnen-3-onegonadotropinnuclear progesterone receptorpituitaryzebrafish

Identifiers

PMID27113852
PMCPMC4938713
OpenAlexW2344689275

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.