Evidence map›Paper›PMID 27113741›Full record

ArticleTumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine2016

A convenient and effective strategy for the enrichment of tumor-initiating cell properties in prostate cancer cells.

Yiming Zhang, Yiqiang Huang, Zhong Jin, Xiezhao Li, Bingkun Li, Peng Xu, Peng Huang, Chunxiao Liu

Abstract read
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In one paragraph

Article in Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed.

  1. Review
  2. Cancer Stem Cells from Definition to Detection and Targeted Drugs.International journal of molecular sciences · 2024
    Review
  3. Cancer Stem Cells and Prostate Cancer: A Narrative Review.International journal of molecular sciences · 2023
    Review
  4. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Yiming ZhangDepartment of Urology, Zhujiang Hospital, Southern Medical University, No. 253 Gongyedadaozhong Road, Haizhu District, Guangzhou, 510282, China.
Yiqiang HuangDepartment of Urology, Zhujiang Hospital, Southern Medical University, No. 253 Gongyedadaozhong Road, Haizhu District, Guangzhou, 510282, China.
Zhong JinDepartment of Urology, Zhujiang Hospital, Southern Medical University, No. 253 Gongyedadaozhong Road, Haizhu District, Guangzhou, 510282, China.
Xiezhao LiDepartment of Urology, Zhujiang Hospital, Southern Medical University, No. 253 Gongyedadaozhong Road, Haizhu District, Guangzhou, 510282, China.
Bingkun LiDepartment of Urology, Zhujiang Hospital, Southern Medical University, No. 253 Gongyedadaozhong Road, Haizhu District, Guangzhou, 510282, China.
Peng XuDepartment of Urology, Zhujiang Hospital, Southern Medical University, No. 253 Gongyedadaozhong Road, Haizhu District, Guangzhou, 510282, China.
Peng HuangDepartment of Urology, Zhujiang Hospital, Southern Medical University, No. 253 Gongyedadaozhong Road, Haizhu District, Guangzhou, 510282, China. professor_huangp@163.com.
Chunxiao LiuDepartment of Urology, Zhujiang Hospital, Southern Medical University, No. 253 Gongyedadaozhong Road, Haizhu District, Guangzhou, 510282, China. dr_liuchunxiao@sina.com.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Stem-like prostate cancer (PrCa) cells, also called PrCa stem cells (PrCSCs) or PrCa tumor-initiating cells (PrTICs), are considered to be involved in the mediation of tumor metastasis and may be responsible for the poor prognosis of PrCa patients. Currently, the methods for PrTIC sorting are mainly based on cell surface marker or side population (SP). However, the rarity of these sorted cells limits the investigation of the molecular mechanisms and therapeutic strategies targeting PrTICs. For PrTIC enrichment, we induced cancer stem cell (CSC) properties in PrCa cells by transducing three defined factors (OCT3/4, SOX2, and KLF4), followed by culture with conventional serum-containing medium. The CSC properties in the transduced cells were evaluated by proliferation, cell cycle, SP assay, drug sensitivity technology, in vivo tumorigenicity, and molecular marker analysis of PrCSCs compared with parental cells and spheroids. After culture with serum-containing medium for 8 days, the PrCa cells transduced with the three factors showed significantly enhanced CSC properties in terms of marker gene expression, sphere formation, chemoresistance to docetaxel, and tumorigenicity. The percentage of CD133

Indexed as

AC133 AntigenAnimalsATP Binding Cassette Transporter, Subfamily G, Member 2Blotting, WesternCell CycleCell Line, TumorCell ProliferationCell Transformation, NeoplasticGene Expression Regulation, NeoplasticHumansHyaluronan ReceptorsKruppel-Like Factor 4Kruppel-Like Transcription FactorsMaleMice, NudeMicroscopy, FluorescenceAC133 AntigenATP Binding Cassette Transporter, Subfamily G, Member 2Hyaluronan ReceptorsKLF4 protein, humanKlf4 protein, mouseKruppel-Like Factor 4Kruppel-Like Transcription FactorsOctamer Transcription Factor-3POU5F1 protein, humanSOX2 protein, humanSOXB1 Transcription FactorsEnrichmentProstate cancerReprogrammingTumor-initiating cells

Identifiers

PMID27113741

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.