ArticleScientific reports2016
Unique behavior of Trypanosoma cruzi mevalonate kinase: A conserved glycosomal enzyme involved in host cell invasion and signaling.
Article in Scientific reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 30 citations in OpenAlex.
- The mevalonate and methylerythritol phosphate pathways as therapeutic targets in protozoan parasites: from moonlighting enzymes to metabolic plasticity.Cell communication and signaling : CCS · 2026Review
- Moonlighting Proteins: Unveiling Their Multifunctionality in Metabolic Regulation and Drug Discovery.Current drug metabolism · 2025Review
- P21 Ablation Unveils Strain-Specific Transcriptional Reprogramming inInternational journal of microbiology · 2025Article
- Metacyclogenesis as the Starting Point of Chagas Disease.International journal of molecular sciences · 2023Review
- A Review on the Immunological Response againstPathogens (Basel, Switzerland) · 2023Review
- Cdc42/Rac Interactive Binding Containing Effector Proteins in Unicellular Protozoans With Reference to Human Host: Locks of the Rho Signaling.Frontiers in genetics · 2022Review
- Released Parasite-Derived Kinases as Novel Targets for Antiparasitic Therapies.Frontiers in cellular and infection microbiology · 2022Review
- Ablation of theFrontiers in cellular and infection microbiology · 2022Article
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- All Roads Lead to Cytosol:Frontiers in cellular and infection microbiology · 2021Review
- Comparative Analysis of Virulence Mechanisms of Trypanosomatids Pathogenic to Humans.Frontiers in cellular and infection microbiology · 2021Review
- Article
- Trypanocidal and leishmanicidal activity of six limonoids.Journal of natural medicines · 2020Article
- Comparative Analysis of the Secretome and Interactome ofFrontiers in immunology · 2020Article
- Functional Peroxisomes Are Essential for Efficient Cholesterol Sensing and Synthesis.Frontiers in cell and developmental biology · 2020Article
- Proteomic analysis of glycosomes from Trypanosoma cruzi epimastigotes.Molecular and biochemical parasitology · 2019Article
- A Carbohydrate Moiety of Secreted Stage-Specific Glycoprotein 4 Participates in Host Cell Invasion byFrontiers in microbiology · 2018Article
- Molecular Characterization ofFrontiers in cellular and infection microbiology · 2018Article
- Amastigote Synapse: The Tricks ofFrontiers in microbiology · 2018Article
- Commentary: Systems Biology Approach to Model the Life Cycle ofFrontiers in cellular and infection microbiology · 2017Article
Corrections and comments
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Authors and funding
14 authors at 4 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Mevalonate kinase (MVK) is an essential enzyme acting in early steps of sterol isoprenoids biosynthesis, such as cholesterol in humans or ergosterol in trypanosomatids. MVK is conserved from bacteria to mammals, and localizes to glycosomes in trypanosomatids. During the course of T. cruzi MVK characterization, we found that, in addition to glycosomes, this enzyme may be secreted and modulate cell invasion. To evaluate the role of TcMVK in parasite-host cell interactions, TcMVK recombinant protein was produced and anti-TcMVK antibodies were raised in mice. TcMVK protein was detected in the supernatant of cultures of metacyclic trypomastigotes (MTs) and extracellular amastigotes (EAs) by Western blot analysis, confirming its secretion into extracellular medium. Recombinant TcMVK bound in a non-saturable dose-dependent manner to HeLa cells and positively modulated internalization of T. cruzi EAs but inhibited invasion by MTs. In HeLa cells, TcMVK induced phosphorylation of MAPK pathway components and proteins related to actin cytoskeleton modifications. We hypothesized that TcMVK is a bifunctional enzyme that in addition to playing a classical role in isoprenoid synthesis in glycosomes, it is secreted and may modulate host cell signaling required for T. cruzi invasion.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.