ArticleScientific reports2016
Human somatic cells subjected to genetic induction with six germ line-related factors display meiotic germ cell-like features.
Article in Scientific reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 26 citations in OpenAlex.
- Initiation of meiosis from human iPSCs under defined conditions through identification of regulatory factors.Science advances · 2025Article
- Induction of Meiosis from Human Pluripotent Stem Cells.bioRxiv : the preprint server for biology · 2024Article
- Assessing data analysis techniques in a high-throughput meiosis-like induction detection system.Plant methods · 2024Article
- A cell-based fluorescent system and statistical framework to detect meiosis-like induction in plants.Frontiers in plant science · 2024Article
- Overcoming roadblocks forFrontiers in plant science · 2023Review
- iPSCs derived from infertile men carrying complex genetic abnormalities can generate primordial germ-like cells.Scientific reports · 2022Article
- Transcriptional control of human gametogenesis.Human reproduction update · 2022Article
- Germ Cell Derivation from Pluripotent Stem Cells for Understanding In Vitro Gametogenesis.Cells · 2021Review
- Human Pluripotent Stem Cells in Reproductive Science-a Comparison of Protocols Used to Generate and Define Male Germ Cells from Pluripotent Stem Cells.International journal of molecular sciences · 2020Review
- Ovarian manipulation in ART: going beyond physiological standards to provide best clinical outcomes.Journal of assisted reproduction and genetics · 2018Review
- Making gametes from alternate sources of stem cells: past, present and future.Reproductive biology and endocrinology : RB&E · 2017Review
- Artificial Sperm: New Horizons in Procreation.Rambam Maimonides medical journal · 2017Review
- CD61 promotes the differentiation of canine ADMSCs into PGC-like cells through modulation of TGF-β signaling.Scientific reports · 2017Article
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Authors and funding
11 authors at 5 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The in vitro derivation of human germ cells has attracted interest in the last years, but their direct conversion from human somatic cells has not yet been reported. Here we tested the ability of human male somatic cells to directly convert into a meiotic germ cell-like phenotype by inducing them with a combination of selected key germ cell developmental factors. We started with a pool of 12 candidates that were reduced to 6, demonstrating that ectopic expression of the germ line-related genes PRDM1, PRDM14, LIN28A, DAZL, VASA and SYCP3 induced direct conversion of somatic cells (hFSK (46, XY), and hMSC (46, XY)) into a germ cell-like phenotype in vitro. Induced germ cell-like cells showed a marked switch in their transcriptomic profile and expressed several post-meiotic germ line related markers, showed meiotic progression, evidence of epigenetic reprogramming, and approximately 1% were able to complete meiosis as demonstrated by their haploid status and the expression of several post-meiotic markers. Furthermore, xenotransplantation assays demonstrated that a subset of induced cells properly colonize the spermatogonial niche. Knowledge obtained from this work can be used to create in vitro models to study gamete-related diseases in humans.
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