Evidence map›Paper›PMID 27112570›Full record

ArticleNucleic acids research2016

Mitochondrial transcription termination factor 1 directs polar replication fork pausing.

Yonghong Shi, Viktor Posse, Xuefeng Zhu, Anne K Hyvärinen, Howard T Jacobs, Maria Falkenberg, Claes M Gustafsson

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Nucleic acids research, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.

0numbers the graph read from it
0cells of the map it votes in
25citing papers in PubMed
2.8field-weighted citation impact, top 10% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

25 citing papers in PubMed, 34 citations in OpenAlex.

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  15. Frontiers in plant science · 2020
    Article
  16. Review
  17. Mechanisms of mammalian mitochondrial transcription.Protein science : a publication of the Protein Society · 2019
    Review
  18. Marek's Disease Virus Infection Induced Mitochondria Changes in Chickens.International journal of molecular sciences · 2019
    Article
  19. Article
  20. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 3 institutions in 3 countries.

Yonghong ShiInstitute of Biomedicine, University of Gothenburg, P.O. Box 440, SE-405 30 Gothenburg, Sweden Center for Molecular Medicine, National Heart Lung and Blood Institute, NIH, Bethesda, MD 20892, USA.
Viktor PosseInstitute of Biomedicine, University of Gothenburg, P.O. Box 440, SE-405 30 Gothenburg, Sweden.
Xuefeng ZhuInstitute of Biomedicine, University of Gothenburg, P.O. Box 440, SE-405 30 Gothenburg, Sweden Center for Molecular Medicine, National Heart Lung and Blood Institute, NIH, Bethesda, MD 20892, USA.
Anne K HyvärinenBioMediTech and Tampere University Hospital, FI-33014, University of Tampere, Finland.
Howard T JacobsBioMediTech and Tampere University Hospital, FI-33014, University of Tampere, Finland Institute of Biotechnology, FI-00014, University of Helsinki, Finland.
Maria FalkenbergInstitute of Biomedicine, University of Gothenburg, P.O. Box 440, SE-405 30 Gothenburg, Sweden claes.gustafsson@medkem.gu.se.
Claes M GustafssonInstitute of Biomedicine, University of Gothenburg, P.O. Box 440, SE-405 30 Gothenburg, Sweden claes.gustafsson@medkem.gu.se.
University of Gothenburg · SETampere University · FITampere University Hospital · FI

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

During replication of nuclear ribosomal DNA (rDNA), clashes with the transcription apparatus can cause replication fork collapse and genomic instability. To avoid this problem, a replication fork barrier protein is situated downstream of rDNA, there preventing replication in the direction opposite rDNA transcription. A potential candidate for a similar function in mitochondria is the mitochondrial transcription termination factor 1 (MTERF1, also denoted mTERF), which binds to a sequence just downstream of the ribosomal transcription unit. Previous studies have shown that MTERF1 prevents antisense transcription over the ribosomal RNA genes, a process which we here show to be independent of the transcription elongation factor TEFM. Importantly, we now demonstrate that MTERF1 arrests mitochondrial DNA (mtDNA) replication with distinct polarity. The effect is explained by the ability of MTERF1 to act as a directional contrahelicase, blocking mtDNA unwinding by the mitochondrial helicase TWINKLE. This conclusion is also supported by in vivo evidence that MTERF1 stimulates TWINKLE pausing. We conclude that MTERF1 can direct polar replication fork arrest in mammalian mitochondria.

Indexed as

DNA ReplicationBasic-Leucine Zipper Transcription FactorsDNA HelicasesDNA, MitochondrialDNA, RibosomalHEK293 CellsHumansMitochondriaMitochondrial ProteinsRNA, RibosomalTranscription FactorsTranscription, GeneticBasic-Leucine Zipper Transcription FactorsDNA HelicasesDNA, MitochondrialDNA, RibosomalMitochondrial ProteinsMTERF1 protein, humanRNA, RibosomalTEFM protein, humanTranscription FactorsTWNK protein, human

Identifiers

PMID27112570
PMCPMC4937320
OpenAlexW2345074464

What OpenQuestion holds

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LicenceCC BY-NC
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.