ArticleNature biotechnology2016
Rapid cloning of disease-resistance genes in plants using mutagenesis and sequence capture.
Article in Nature biotechnology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 206 papers, 1 of them a synthesis that pooled it.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
206 citing papers in PubMed, 1 synthesis or guideline pooled it, 432 citations in OpenAlex.
- Unravelling consensus genomic regions conferring stem-related traits in wheat via meta-QTL analysis.BMC plant biology · 2025Pooled it
- Genome-wide prediction of cloned disease resistance homologues across the Oryza genus.The plant genome · 2026Article
- No Cost of Resistance in Wheat Gene Stack Lines Containing 10 Stem Rust Resistance Transgenes.Plant biotechnology journal · 2026Article
- Beyond R-Genes: Dissecting Metabolic and Nutrient-Driven Wheat Rust Resistance Through Induced Mutagenesis.Plants (Basel, Switzerland) · 2026Review
- Characterization of Durum-Einkorn Amphiploids for Introgression of Powdery Mildew Resistance from Einkorn into Common Wheat.Pathogens (Basel, Switzerland) · 2026Article
- Advances in gene cloning and functional genomics approaches for wheat (Triticum aestivum L.) improvement.The plant genome · 2026Review
- WheatRPred: deep learning framework for predicting disease resistance protein in wheat and its wild relatives.Journal, genetic engineering & biotechnology · 2026Article
- Comparative transcriptome analysis identifies key players associated with downy mildew resistance in foxtail millet variety JG21 and its resistant mutant rdm12.BMC plant biology · 2026Article
- Genomics in wheat improvement: Progress and perspectives.The plant genome · 2026Review
- Accelerating Resistance Breeding: Emerging Methods to Identify and Validate Plant Immunity Genes.Plants (Basel, Switzerland) · 2026Review
- Targeted capture and cloning of Rps6 reveal redundancy in soybean resistance genes.BMC plant biology · 2026Article
- Pm37 as a susceptible Sr22 allele confers resistance to wheat powdery mildew and leaf rust.Nature communications · 2026Article
- INDETERMINATE DOMAIN-DELLA protein interactions orchestrate gibberellin-mediated cell elongation in wheat and barley.Proceedings of the National Academy of Sciences of the United States of America · 2026Article
- Genome-based mutant RNA mapping identifies an NLR pair underlying Yr6-mediated stripe rust resistance in wheat.Plant communications · 2026Article
- From gene-for-gene to NLRome-for-effectorome: decoding the dynamic interplay between phytopathogenic effector networks and plant immune systems.FEMS microbiology reviews · 2026Review
- Article
- The Application of RenSeq for Pan-NLRome Analysis.Methods in molecular biology (Clifton, N.J.) · 2026Article
- Harnessing primary, secondary and tertiary genepools for durable wheat disease resistance.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2025Review
- Discovery of functional NLRs using expression level, high-throughput transformation and large-scale phenotyping.Nature plants · 2025Article
- Text mining reveals the increasing importance of genomic approaches in crop research and breeding.Molecular breeding : new strategies in plant improvement · 2025Review
146 more citing papers are in PubMed but not listed here.
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Authors and funding
12 authors at 5 institutions in 4 countries.
Funding
Abstract
Wild relatives of domesticated crop species harbor multiple, diverse, disease resistance (R) genes that could be used to engineer sustainable disease control. However, breeding R genes into crop lines often requires long breeding timelines of 5-15 years to break linkage between R genes and deleterious alleles (linkage drag). Further, when R genes are bred one at a time into crop lines, the protection that they confer is often overcome within a few seasons by pathogen evolution. If several cloned R genes were available, it would be possible to pyramid R genes in a crop, which might provide more durable resistance. We describe a three-step method (MutRenSeq)-that combines chemical mutagenesis with exome capture and sequencing for rapid R gene cloning. We applied MutRenSeq to clone stem rust resistance genes Sr22 and Sr45 from hexaploid bread wheat. MutRenSeq can be applied to other commercially relevant crops and their relatives, including, for example, pea, bean, barley, oat, rye, rice and maize.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.