ArticleBritish journal of pharmacology2016
Antagonizing midkine accelerates fracture healing in mice by enhanced bone formation in the fracture callus.
Article in British journal of pharmacology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 31 citations in OpenAlex.
- Targeting MDK alleviates bone loss via dual regulation of osteogenic differentiation and inflammatory cytokine expression.Genes & diseases · 2026Article
- Integrating Single-Cell and Microarray Data to Explore the Role of Autophagy-Related Gene Atg7 in Osteoporosis.Journal of inflammation research · 2026Article
- The influence of adrenoceptor blocker treatment on fracture healing in osteoporotic and non-osteoporotic bone.Frontiers in physiology · 2025Article
- Therapeutic approaches to activate the canonical Wnt pathway for bone regeneration.Journal of tissue engineering and regenerative medicine · 2022Review
- Osteoblast lineage Sod2 deficiency leads to an osteoporosis-like phenotype in mice.Disease models & mechanisms · 2022Article
- Systemic and local cardiac inflammation after experimental long bone fracture, traumatic brain injury and combined trauma in mice.Journal of orthopaedic translation · 2021Article
- Differences in Fracture Healing Between Female and Male C57BL/6J Mice.Frontiers in physiology · 2021Article
- Effects of Estrogen Receptor and Wnt Signaling Activation on Mechanically Induced Bone Formation in a Mouse Model of Postmenopausal Bone Loss.International journal of molecular sciences · 2020Article
- Pregnancy and lactation, a challenge for the skeleton.Endocrine connections · 2020Review
- Midkine Is Elevated After Multiple Trauma and Acts Directly on Human Cardiomyocytes by Altering Their Functionality and Metabolism.Frontiers in immunology · 2019Article
- Influence of Menopause on Inflammatory Cytokines during Murine and Human Bone Fracture Healing.International journal of molecular sciences · 2018Article
- The inflammatory phase of fracture healing is influenced by oestrogen status in mice.European journal of medical research · 2017Article
- Antagonizing midkine accelerates fracture healing in mice by enhanced bone formation in the fracture callus.British journal of pharmacology · 2016Article
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Authors and funding
8 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
background and purposePrevious findings suggest that the growth and differentiation factor midkine (Mdk) is a negative regulator of osteoblast activity and bone formation, thereby raising the possibility that a specific Mdk antagonist might improve bone formation during fracture healing. EXPERIMENTAL APPROACH: In the present study, we investigated the effects of a monoclonal anti-Mdk antibody (Mdk-Ab) on bone healing using a standardized femur osteotomy model in mice. Additional in vitro experiments using chondroprogenitor and preosteoblastic cells were conducted to analyse the effects of recombinant Mdk and Mdk-Ab on differentiation markers and potential binding partners in these cells. KEY
resultsWe demonstrated that treatment with Mdk-Ab accelerated bone healing in mice based on increased bone formation in the fracture callus. In vitro experiments using preosteoblastic cells showed that Mdk-Ab treatment abolished the Mdk-induced negative effects on the expression of osteogenic markers and Wnt/β-catenin target proteins, whereas the differentiation of chondroprogenitor cells was unaffected. Phosphorylation analyses revealed an important role for the low-density lipoproteinLDL receptor-related protein 6 in Mdk signalling in osteoblasts. CONCLUSIONS AND IMPLICATIONS: We conclude that Mdk-Ab treatment may be a potential novel therapeutic strategy to enhance fracture healing in patients with orthopaedic complications such as delayed healing or non-union formation.
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