ArticleACS synthetic biology2016
Efflux Pump Control Alters Synthetic Gene Circuit Function.
Article in ACS synthetic biology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
What it found
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Who cites it
9 citing papers in PubMed.
- Efflux pump knockout minimizes crosstalk and boosts response in transcription factor-based biosensors.Journal of biological engineering · 2025Article
- The Growth Dependent Design Constraints of Transcription-Factor-Based Metabolite Biosensors.ACS synthetic biology · 2022Article
- Mathematical Modelling Highlights the Potential for Genetic Manipulation as an Adjuvant to Counter Efflux-Mediated MDR in Salmonella.Bulletin of mathematical biology · 2022Article
- Time dependent asymptotic analysis of the gene regulatory network of the AcrAB-TolC efflux pump system in gram-negative bacteria.Journal of mathematical biology · 2021Article
- Macrolide Biosensor Optimization through Cellular Substrate Sequestration.ACS synthetic biology · 2021Article
- Observation and Control of Gene Expression Noise: Barrier Crossing Analogies Between Drug Resistance and Metastasis.Frontiers in genetics · 2020Article
- Advancing Antimicrobial Resistance Research Through Quantitative Modeling and Synthetic Biology.Frontiers in bioengineering and biotechnology · 2020Article
- Multiscale effects of heating and cooling on genes and gene networks.Proceedings of the National Academy of Sciences of the United States of America · 2018Article
- Negative Regulation Gene Circuits for Efflux Pump Control.Methods in molecular biology (Clifton, N.J.) · 2018Article
Corrections and comments
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Authors and funding
6 authors.
Funding
Abstract
Synthetic biology aims to design new biological systems for predefined purposes, such as the controlled secretion of biofuels, pharmaceuticals, or other chemicals. Synthetic gene circuits regulating an efflux pump from the ATP-binding cassette (ABC) protein family could achieve this. However, ABC efflux pumps can also drive out intracellular inducer molecules that control the gene circuits. This will introduce an implicit feedback that could alter gene circuit function in ways that are poorly understood. Here, we used two synthetic gene circuits inducible by tetracycline family molecules to regulate the expression of a yeast ABC pump (Pdr5p) that pumps out the inducer. Pdr5p altered the dose-responses of the original gene circuits substantially in Saccharomyces cerevisiae. While one aspect of the change could be attributed to the efflux pumping function of Pdr5p, another aspect remained unexplained. Quantitative modeling indicated that reduced regulator gene expression in addition to efflux pump function could fully explain the altered dose-responses. These predictions were validated experimentally. Overall, we highlight how efflux pumps can alter gene circuit dynamics and demonstrate the utility of mathematical modeling in understanding synthetic gene circuit function in new circumstances.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.