ArticleCurrent protocols in molecular biology
Single-Molecule mRNA Detection in Live Yeast.
Article in Current protocols in molecular biology. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 35 citations in OpenAlex.
- Current practices in the study of biomolecular condensates: a community comment.Nature communications · 2025Article
- Cell-cycle dependence of bursty gene expression: insights from fitting mechanistic models to single-cell RNA-seq data.Nucleic acids research · 2025Article
- Transient power-law behaviour following induction distinguishes between competing models of stochastic gene expression.Nature communications · 2025Article
- Contributions of transcriptional noise to leukaemia evolution: KAT2A as a case-study.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2024Review
- Disordered C-terminal domain drives spatiotemporal confinement of RNAPII to enhance search for chromatin targets.Nature cell biology · 2024Article
- Quantifying and correcting bias in transcriptional parameter inference from single-cell data.Biophysical journal · 2024Article
- Disordered C-terminal domain drives spatiotemporal confinement of RNAPII to enhance search for chromatin targets.bioRxiv : the preprint server for biology · 2023Article
- Transcription factor clusters enable target search but do not contribute to target gene activation.Nucleic acids research · 2023Article
- DNA supercoiling restricts the transcriptional bursting of neighboring eukaryotic genes.Molecular cell · 2023Article
- Inducible transcriptional condensates drive 3D genome reorganization in the heat shock response.Molecular cell · 2022Article
- Article
- Long-term imaging of individual mRNA molecules in living cells.Cell reports methods · 2022Article
- Nuclear envelope mechanobiology: linking the nuclear structure and function.Nucleus (Austin, Tex.) · 2021Review
- The Hda1 histone deacetylase limits divergent non-coding transcription and restricts transcription initiation frequency.The EMBO journal · 2021Article
- CATS: Cas9-assisted tag switching. A high-throughput method for exchanging genomic peptide tags in yeast.BMC genomics · 2020Article
- Using measures of single-cell physiology and physiological state to understand organismic aging.Aging cell · 2016Review
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Visualization of single RNA molecules in living cells has enabled the study of synthesis, movement, and localization of mRNAs and has provided insight into gene regulation with sub-second temporal resolution and nanometer spatial resolution. Following transcription in single cells indicates that gene activity is heterogeneous between cells and also exhibits random variability over time even within single cells. Studies of mRNAs in yeast can take advantage of the powerful genetics available in this model organism and allow mechanistic questions to be addressed. In this chapter, we describe an approach for visualizing mRNA and transcription in live yeast cells. The method is based on binding of fluorescently labeled MS2 and PP7 coat proteins to stem loops sequences that are introduced into the gene of interest. We give detailed protocols for the construction of the necessary yeast strains, for image acquisition, and for validation.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.