ArticleFrontiers in zoology2016
Skeletal regeneration in the brittle star Amphiura filiformis.
Article in Frontiers in zoology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed, 55 citations in OpenAlex.
- Chromosome-level genome assembly and single-cell analysis unveil molecular mechanisms of arm regeneration in the ophiuroid Ophiura sarsii vadicola.Genome biology · 2025Article
- Availability and occurrence of coelenterazine in a Swedish fjord to maintain Amphiura filiformis bioluminescence.Scientific reports · 2024Article
- The brittle star genome illuminates the genetic basis of animal appendage regeneration.Nature ecology & evolution · 2024Article
- Origins of cancer: ain't it just mature cells misbehaving?The EMBO journal · 2024Review
- Bio-inspired compensatory strategies for damage to flapping robotic propulsors.Journal of the Royal Society, Interface · 2024Article
- Tissue Extract from Brittle Star Undergoing Arm Regeneration Promotes Wound Healing in Rat.Marine drugs · 2023Article
- EchinoDB: an update to the web-based application for genomic and transcriptomic data on echinoderms.BMC genomic data · 2022Article
- Neurogenesis during Brittle Star Arm Regeneration Is Characterised by a Conserved Set of Key Developmental Genes.Biology · 2022Article
- A subterminal growth zone at arm tip likely underlies life-long indeterminate growth in brittle stars.Frontiers in zoology · 2022Article
- The Evolution of Biomineralization through the Co-Option of Organic Scaffold Forming Networks.Cells · 2022Review
- A pan-metazoan concept for adult stem cells: the wobbling Penrose landscape.Biological reviews of the Cambridge Philosophical Society · 2022Article
- Studying Echinodermata Arm Explant Regeneration Using Echinaster sepositus.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Review
- FGF signalling plays similar roles in development and regeneration of the skeleton in the brittle star Amphiura filiformis.Development (Cambridge, England) · 2021Article
- Post-metamorphic skeletal growth in the sea urchin Paracentrotus lividus and implications for body plan evolution.EvoDevo · 2021Article
- Ultrastructural and molecular analysis of the origin and differentiation of cells mediating brittle star skeletal regeneration.BMC biology · 2021Article
- Regeneration in Echinoderms: Molecular Advancements.Frontiers in cell and developmental biology · 2021Review
- Article
- Beyond Adult Stem Cells: Dedifferentiation as a Unifying Mechanism Underlying Regeneration in Invertebrate Deuterostomes.Frontiers in cell and developmental biology · 2020Review
- The complete mitochondrial genome of a deep sea ophiuroid of the genusMitochondrial DNA. Part B, Resources · 2019Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
Abstract
backgroundBrittle stars regenerate their whole arms post-amputation. Amphiura filiformis can now be used for molecular characterization of arm regeneration due to the availability of transcriptomic data. Previous work showed that specific developmental transcription factors known to take part in echinoderm skeletogenesis are expressed during adult arm regeneration in A. filiformis; however, the process of skeleton formation remained poorly understood. Here, we present the results of an in-depth microscopic analysis of skeletal morphogenesis during regeneration, using calcein staining, EdU labeling and in situ hybridization.
resultsTo better compare different samples, we propose a staging system for the early A. filiformis arm regeneration stages based on morphological landmarks identifiable in living animals and supported by histological analysis. We show that the calcified spicules forming the endoskeleton first appear very early during regeneration in the dermal layer of regenerates. These spicules then mature into complex skeletal elements of the differentiated arm during late regeneration. The mesenchymal cells in the dermal area express the skeletal marker genes Afi-c-lectin, Afi-p58b and Afi-p19; however, EdU labeling shows that these dermal cells do not proliferate.
conclusionsA. filiformis arms regenerate through a consistent set of developmental stages using a distalization-intercalation mode, despite variability in regeneration rate. Skeletal elements form in a mesenchymal cell layer that does not proliferate and thus must be supplied from a different source. Our work provides the basis for future cellular and molecular studies of skeleton regeneration in brittle stars.
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