Evidence map›Paper›PMID 27110245›Full record

ArticleInternational journal of microbiology2016

IncF Plasmids Are Commonly Carried by Antibiotic Resistant Escherichia coli Isolated from Drinking Water Sources in Northern Tanzania.

Beatus Lyimo, Joram Buza, Murugan Subbiah, Sylivester Temba, Honest Kipasika, Woutrina Smith, Douglas R Call

Open access · goldAbstract read
In one paragraph

Article in International journal of microbiology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.

0numbers the graph read from it
0cells of the map it votes in
32citing papers in PubMed
3.0field-weighted citation impact, top 10% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

32 citing papers in PubMed, 70 citations in OpenAlex.

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  11. Antimicrobial resistance in food-associatedBioscience of microbiota, food and health · 2024
    Review
  12. Article
  13. High Frequency of Antibiotic Resistance Genes (ARGs) in the Lerma River Basin, Mexico.International journal of environmental research and public health · 2022
    Article
  14. Article
  15. Article
  16. Article
  17. Article
  18. Review
  19. Article
  20. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 3 institutions in 2 countries.

Beatus LyimoNelson Mandela African Institution of Science and Technology, 447 Arusha, Tanzania.
Joram BuzaNelson Mandela African Institution of Science and Technology, 447 Arusha, Tanzania.
Murugan SubbiahPaul G. Allen School for Global Animal Health, Washington State University, Pullman, WA 99164, USA.
Sylivester TembaNelson Mandela African Institution of Science and Technology, 447 Arusha, Tanzania.
Honest KipasikaNelson Mandela African Institution of Science and Technology, 447 Arusha, Tanzania.
Woutrina SmithOne Health Institute, School of Veterinary Medicine, University of California, Davis, CA 95616, USA.
Douglas R CallNelson Mandela African Institution of Science and Technology, 447 Arusha, Tanzania; Paul G. Allen School for Global Animal Health, Washington State University, Pullman, WA 99164, USA.
Nelson Mandela African Institution of Science and Technology · TZUniversity of California, Davis · USWashington State University · US

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The aim of this study was to identify the replicon types of plasmids, conjugation efficiencies, and the complement of antibiotic resistance genes for a panel of multidrug resistant E. coli isolates from surface waters in northern Tanzania. Standard membrane filtration was used to isolate and uidA PCR was used to confirm the identity of strains as E. coli. Antibiotic susceptibility was determined by breakpoint assay and plasmid conjugation was determined by filter-mating experiments. PCR and sequencing were used to identify resistance genes and PCR-based replicon typing was used to determine plasmid types. Filter mating experiments indicated conjugation efficiencies ranged from 10(-1) to 10(-7). Over 80% of the donor cells successfully passed their resistance traits and eleven different replicon types were detected (IncI1, FIC, P, FIIA, A/C, FIB, FIA, H12, K/B B/O, and N). IncF plasmids were most commonly detected (49% of isolates), followed by types IncI1 and IncA/C. Detection of these public health-relevant conjugative plasmids and antibiotic resistant traits in Tanzanian water suggests the possible pollution of these water sources from human, livestock, and wild animal wastes and also shows the potential of these water sources in the maintenance and transmission of these resistance traits between environments, animals, and people.

Identifiers

PMID27110245
PMCPMC4823495
OpenAlexW2305973580

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.