ArticleScientific reports2016
Identification of residues crucial for the interaction between human neuroglobin and the α-subunit of heterotrimeric Gi protein.
Article in Scientific reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.
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5 citing papers in PubMed, 10 citations in OpenAlex.
- Neuroglobin Expression in the Brain: a Story of Tissue Homeostasis Preservation.Molecular neurobiology · 2019Review
- Investigating molecular interactions between oxidized neuroglobin and cytochrome c.Scientific reports · 2018Article
- Chimeric ZHHH neuroglobin acts as a cell membrane-penetrating inducer of neurite outgrowth.FEBS open bio · 2017Article
- Hemoglobin-Improved Protection in Cultured Cerebral Cortical Astroglial Cells: Inhibition of Oxidative Stress and Caspase Activation.Frontiers in endocrinology · 2017Article
- Neuroglobin, a Factor Playing for Nerve Cell Survival.International journal of molecular sciences · 2016Review
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
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Abstract
Mammalian neuroglobin (Ngb) protects neuronal cells under conditions of oxidative stress. We previously showed that human Ngb acts as a guanine nucleotide dissociation inhibitor (GDI) for the α-subunits of heterotrimeric Gi/o proteins and inhibits the decrease in cAMP concentration, leading to protection against cell death. In the present study, we used an eukaryotic expression vector driving high-level expression of human wild-type Ngb or Ngb mutants that either exhibit or lack GDI activities in human cells. We demonstrate that the GDI activity of human Ngb is tightly correlated with its neuroprotective activity. We further demonstrate that Glu53, Glu60, and Glu118 of human Ngb are crucial for both the neuroprotective activity and interaction with Gαi1. Moreover, we show that Lys46, Lys70, Arg208, Lys209, and Lys210 residues of Gαi1 are important for binding to human Ngb. We propose a molecular docking model of the complex between human Ngb and Gαi1.
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