ArticleScientific reports2016
Engineering a d-lactate dehydrogenase that can super-efficiently utilize NADPH and NADH as cofactors.
Article in Scientific reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 36 citations in OpenAlex.
- Enhancing prediction accuracy for enzyme activity engineering through sequence co-evolution and epistatic relationship modeling.Nucleic acids research · 2026Article
- Cofactor Engineering Strategy of Food-Grade Microorganisms: Redox Homeostasis Regulation and Functional Components Biofortification.Microorganisms · 2026Review
- Programmable Enzymatic Reaction Network in Artificial Cell-Like Polymersomes.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2024Article
- Key therapeutic targets implicated at the early stage of hepatocellular carcinoma identified through machine-learning approaches.Scientific reports · 2023Article
- Biological characterization of D-lactate dehydrogenase responsible for high-yield production of D-phenyllactic acid in Sporolactobacillus inulinus.Microbial biotechnology · 2022Article
- Solid-Binding Peptide-Guided Spatially Directed Immobilization of Kinetically Matched Enzyme Cascades in Membrane Nanoreactors.ACS omega · 2021Article
- Over-expression of an electron transport protein OmcS provides sufficient NADH for D-lactate production in cyanobacterium.Biotechnology for biofuels · 2021Article
- Enhanced Production of D-Lactate in Cyanobacteria by Re-Routing Photosynthetic Cyclic and Pseudo-Cyclic Electron Flow.Frontiers in plant science · 2019Article
- Steric hindrance controls pyridine nucleotide specificity of a flavin-dependent NADH:quinone oxidoreductase.Protein science : a publication of the Protein Society · 2019Article
- Development of a High-Throughput, In Vivo Selection Platform for NADPH-Dependent Reactions Based on Redox Balance Principles.ACS synthetic biology · 2018Article
- Prognostic value of D-lactate dehydrogenase in patients with clear cell renal cell carcinoma.Oncology letters · 2018Article
- Redox cofactor engineering in industrial microorganisms: strategies, recent applications and future directions.Journal of industrial microbiology & biotechnology · 2018Review
- Substrate Specificity and Allosteric Regulation of a D-Lactate Dehydrogenase from a Unicellular Cyanobacterium are Altered by an Amino Acid Substitution.Scientific reports · 2017Article
- Engineering redox homeostasis to develop efficient alcohol-producing microbial cell factories.Microbial cell factories · 2017Review
- Coenzyme Engineering of a Hyperthermophilic 6-Phosphogluconate Dehydrogenase from NADPScientific reports · 2016Article
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Authors and funding
7 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Engineering the cofactor specificity of a natural enzyme often results in a significant decrease in its activity on original cofactor. Here we report that a NADH-dependent dehydrogenase (d-LDH) from Lactobacillus delbrueckii 11842 can be rationally engineered to efficiently use both NADH and NADPH as cofactors. Point mutations on three amino acids (D176S, I177R, F178T) predicted by computational analysis resulted in a modified enzyme designated as d-LDH*. The Kcat/Km of the purified d-LDH* on NADPH increased approximately 184-fold while the Kcat/Km on NADH also significantly increased, showing for the first time that a rationally engineered d-LDH could exhibit comparable activity on both NADPH and NADH. Further kinetic analysis revealed that the enhanced affinity with NADH or NADPH and the significant increased Kcat of d-LDH* resulted in the significant increase of d-LDH* activity on both NADPH and NADH. This study thus demonstrated that the cofactor specificity of dehydrogenase can be broadened by using targeted engineering approach, and the engineered enzyme can efficiently function in NADH-rich, or NADPH-rich, or NADH and NADPH-rich environment.
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