ArticleMolecules and cells2016
Overexpression of Heat Shock Factor Gene HsfA3 Increases Galactinol Levels and Oxidative Stress Tolerance in Arabidopsis.
Article in Molecules and cells, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 46 papers, 1 of them a synthesis that pooled it.
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46 citing papers in PubMed, 1 synthesis or guideline pooled it, 81 citations in OpenAlex.
- Genome-wide transcriptional analysis of rapeseed (Brassica napus L.) responses to heat stress: an integrated RNA-Seq meta-analysis, gene co-expression, and gene regulatory network study.BMC plant biology · 2025Pooled it
- Physiology and Transcriptomics Reveal Divergent Strategies of Mycorrhiza-Mediated Drought Adaptation in Poplar.Plant, cell & environment · 2026Article
- Exogenous application of γ-aminobutyric acid alleviates temperature stress in mungbean (Vigna radiata) and its wild non-progenitor (Vigna glabrescens) by regulating heat shock protein genes.BMC plant biology · 2025Article
- Conserved heat shock factors HvHSFA2 and HvHSFA3 control barley heat stress memory through diverged mechanisms.Nature communications · 2025Article
- MdHSFA2 enhances heat stress tolerance through promoting the MdGolS4 transcription in apple.Scientific reports · 2025Article
- Evolution of drought resistance strategies following the introduction of white clover (Trifolium repens L.).Annals of botany · 2025Article
- Transcriptomic Identification of Key Genes Responding to High Heat Stress in Moso Bamboo (Genes · 2025Article
- The bZIP transcription factor CibZIP43 positively regulates salt and heat tolerance in pecan (Carya illinoinensis).BMC plant biology · 2025Article
- Priming thermotolerance: unlocking heat resilience for climate-smart crops.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2025Review
- Characterization of the heat shock factor RcHsfA6 in Rosa chinensis and function in the thermotolerance of Arabidopsis.BMC plant biology · 2025Article
- High concentration of phosphate treatment increased the tolerance of Robinia pseudoacacia roots to salt stress.Plant cell reports · 2025Article
- The CRY1-HsF predicted interaction interface serves as a molecular platform for bioengineering or selecting modulating mutants.Frontiers in plant science · 2025Article
- Genome-Wide Analysis of the Hsf Gene Family inInternational journal of molecular sciences · 2024Article
- Transcriptomic and physiological analyses reveal different grape varieties response to high temperature stress.Frontiers in plant science · 2024Article
- Comparative Physiological and Transcriptome Analyses of Tolerant and Susceptible Cultivars Reveal the Molecular Mechanism of Cold Tolerance inInternational journal of molecular sciences · 2023Article
- Genome-Wide Identification ofInternational journal of molecular sciences · 2023Article
- Article
- Transcriptome Analysis Reveals Differentially Expressed Genes Involved in Cadmium and Arsenic Accumulation in Tea Plant (Plants (Basel, Switzerland) · 2023Article
- Molecular Mechanism of Cold Tolerance of Centipedegrass Based on the Transcriptome.International journal of molecular sciences · 2023Article
- Hsf transcription factor gene family in peanut (Frontiers in plant science · 2023Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
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Abstract
Heat shock factors (Hsfs) are central regulators of abiotic stress responses, especially heat stress responses, in plants. In the current study, we characterized the activity of the Hsf gene HsfA3 in Arabidopsis under oxidative stress conditions. HsfA3 transcription in seedlings was induced by reactive oxygen species (ROS), exogenous hydrogen peroxide (H2O2), and an endogenous H2O2 propagator, 2,5-dibromo-3-methyl-6-isopropyl-p-benzoquinone (DBMIB). HsfA3-overexpressing transgenic plants exhibited increased oxidative stress tolerance compared to untransformed wild-type plants (WT), as revealed by changes in fresh weight, chlorophyll fluorescence, and ion leakage under light conditions. The expression of several genes encoding galactinol synthase (GolS), a key enzyme in the biosynthesis of raffinose family oligosaccharides (RFOs), which function as antioxidants in plant cells, was induced in HsfA3 overexpressors. In addition, galactinol levels were higher in HsfA3 overexpressors than in WT under unstressed conditions. In transient transactivation assays using Arabidopsis leaf protoplasts, HsfA3 activated the transcription of a reporter gene driven by the GolS1 or GolS2 promoter. Electrophoretic mobility shift assays showed that GolS1 and GolS2 are directly regulated by HsfA3. Taken together, these findings provide evidence that GolS1 and GolS2 are directly regulated by HsfA3 and that GolS enzymes play an important role in improving oxidative stress tolerance by increasing galactinol biosynthesis in Arabidopsis.
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