ArticleThe Journal of veterinary medical science2016
An improved loop-mediated isothermal amplification assay for the detection of Mycoplasma bovis.
Article in The Journal of veterinary medical science, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed.
- Synergistic Integration of Recombinase Polymerase Amplification and CRISPR-Cas12a Enables High-Sensitivity Detection of Mycoplasma bovis.MicrobiologyOpen · 2026Article
- Bovine Respiratory Mycoplasmas and the Commensal-Pathogen Continuum: A Systematic Review of Vaccines and Diagnostic Approaches.Animals : an open access journal from MDPI · 2026Review
- Development of primers for loop-mediated isothermal amplification for Mycoplasma canadense detection.The Journal of veterinary medical science · 2025Article
- Point-of-care potentials of lateral flow-based field screening forBiology methods & protocols · 2024Review
- Review
- A Systematic Review of the Recent Techniques Commonly Used in the Diagnosis ofPathogens (Basel, Switzerland) · 2023Review
- A novel polymerase chain reaction assay for the detection of seven Mycoplasma species of cattle origin.World journal of microbiology & biotechnology · 2022Article
- Bovine Respiratory Disease: Conventional to Culture-Independent Approaches to Studying Antimicrobial Resistance in North America.Antibiotics (Basel, Switzerland) · 2022Review
- Production of granulomas inJournal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc · 2022Article
- Direct and Rapid Detection ofFrontiers in cellular and infection microbiology · 2021Article
- Combination of procedure for ultra rapid extraction (PURE) and loop-mediated isothermal amplification (LAMP) for rapid detection of Mycoplasma bovis in milk.The Journal of veterinary medical science · 2020Article
- Upregulation of PD-L1 Expression by Prostaglandin EFrontiers in veterinary science · 2020Article
- Development and validation of a loop-mediated isothermal amplification assay for the detection of Mycoplasma bovis in mastitic milk.Folia microbiologica · 2018Article
- The presence of adhesion factors NOX, α-enolase, TrmFO, P27, and VpmaX inOpen veterinary journalArticle
Corrections and comments
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We improved a loop-mediated isothermal amplification (LAMP) assay permitting sensitive and rapid Mycoplasma bovis detection. A total of 55 bacterial strains were examined in this study, including 33 M. bovis strains, 14 non-M. bovis mycoplasmas and eight non-mycoplasma bacterial strains. M. bovis was successfully detected by the LAMP assay within 60 min without cross-reaction to any other bacteria. Furthermore, a total of 135 nasal swab samples were tested directly using our LAMP assays, the previously reported LAMP assay, conventional PCR assay without pre-culture and comparing standard culture methods. The improved LAMP assay showed sensitivity and specificity of 97.2% and 90.9%, respectively (with a kappa coefficient of 0.8231), and the sensitivity of our revised LAMP assay was increased compared to existing methods.
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