ArticleScientific reports2016
Generation of transgenic cynomolgus monkeys that express green fluorescent protein throughout the whole body.
Article in Scientific reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers, 1 of them a synthesis that pooled it.
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Who cites it
30 citing papers in PubMed, 1 synthesis or guideline pooled it, 37 citations in OpenAlex.
- Large animals in neurointerventional research: A systematic review on models, techniques and their application in endovascular procedures for stroke, aneurysms and vascular malformations.Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism · 2019Pooled it
- Non-viral generation of transgenic non-human primates via the piggyBac transposon system.Nature communications · 2025Article
- Liver-specific transgenic expression of human NTCP in rhesus macaques confers HBV susceptibility on primary hepatocytes.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Non-human primate: the new frontier model of female reproductive engineering.Frontiers in bioengineering and biotechnology · 2025Review
- Comparative analysis of superovulated versus uterine-embryo synchronized recipients for embryo transfer in cynomolgus monkeys (Frontiers in veterinary science · 2024Article
- Generation of a familial hypercholesterolemia model in non-human primate.Scientific reports · 2023Article
- Article
- Screening for gene doping transgenes in horses via the use of massively parallel sequencing.Gene therapy · 2022Article
- Modeling genetic diseases in nonhuman primates through embryonic and germline modification: Considerations and challenges.Science translational medicine · 2022Review
- Gene editing monkeys: Retrospect and outlook.Frontiers in cell and developmental biology · 2022Review
- Non-human primates as a model for human development.Stem cell reports · 2021Review
- Generation and Breeding ofCells · 2021Article
- Embryo-Engineered Nonhuman Primate Models: Progress and Gap to Translational Medicine.Research (Washington, D.C.) · 2021Review
- Assisted Reproductive Techniques and Genetic Manipulation in the Common Marmoset.ILAR journal · 2020Review
- Generation of Transgenic Cynomolgus Monkeys Overexpressing the Gene for Amyloid-β Precursor Protein.Journal of Alzheimer's disease : JAD · 2020Article
- Monkeys mutant for PKD1 recapitulate human autosomal dominant polycystic kidney disease.Nature communications · 2019Article
- Highly efficient induction of primate iPS cells by combining RNA transfection and chemical compounds.Genes to cells : devoted to molecular & cellular mechanisms · 2019Article
- Poor second ovarian stimulation in cynomolgus monkeys (Macaca fascicularis) is associated with the production of antibodies against human follicle-stimulating hormone.The Journal of reproduction and development · 2019Article
- Hyperandrogenic origins of polycystic ovary syndrome - implications for pathophysiology and therapy.Expert review of endocrinology & metabolism · 2019Review
- Local transgene expression and whole-body transgenesis to model brain diseases in nonhuman primate.Animal models and experimental medicine · 2019Review
Corrections and comments
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Authors and funding
19 authors at 6 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Nonhuman primates are valuable for human disease modelling, because rodents poorly recapitulate some human diseases such as Parkinson's disease and Alzheimer's disease amongst others. Here, we report for the first time, the generation of green fluorescent protein (GFP) transgenic cynomolgus monkeys by lentivirus infection. Our data show that the use of a human cytomegalovirus immediate-early enhancer and chicken beta actin promoter (CAG) directed the ubiquitous expression of the transgene in cynomolgus monkeys. We also found that injection into mature oocytes before fertilization achieved homogenous expression of GFP in each tissue, including the amnion, and fibroblasts, whereas injection into fertilized oocytes generated a transgenic cynomolgus monkey with mosaic GFP expression. Thus, the injection timing was important to create transgenic cynomolgus monkeys that expressed GFP homogenously in each of the various tissues. The strategy established in this work will be useful for the generation of transgenic cynomolgus monkeys for transplantation studies as well as biomedical research.
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