ArticleDiagnostic microbiology and infectious disease2016
Simultaneous detection of Legionella species and L. anisa, L. bozemanii, L. longbeachae and L. micdadei using conserved primers and multiple probes in a multiplex real-time PCR assay.
Article in Diagnostic microbiology and infectious disease, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
14 citing papers in PubMed, 29 citations in OpenAlex.
- MixedInfectious disease reports · 2026Article
- Development of a PCR Assay for the Detection ofInfectious disease reports · 2025Article
- Case Report: First report ofFrontiers in immunology · 2025Article
- The Contribution ofInternational journal of environmental research and public health · 2024Article
- Opportunistic Pathogens in Drinking Water Distribution Systems-A Review.Microorganisms · 2024Review
- Mysteriously rapid rise in Legionnaires' disease incidence correlates with declining atmospheric sulfur dioxide.PNAS nexus · 2024Article
- Inflammatory Patterns Associated withPathogens (Basel, Switzerland) · 2024Article
- New immunomagnetic separation method to analyze risk factors for Legionella colonization in health care centres.Journal of exposure science & environmental epidemiology · 2022Article
- Development of a Novel Peptide Nucleic Acid Probe for the Detection ofMicroorganisms · 2022Article
- Water Age Effects on the Occurrence and Concentration ofMicroorganisms · 2021Article
- Cooccurrence of Five PathogenicPathogens (Basel, Switzerland) · 2021Article
- Optimising the case-crossover design for use in shared exposure settings.Epidemiology and infection · 2020Article
- Short-term associations between Legionnaires' disease incidence and meteorological variables in Belgium, 2011-2019.Epidemiology and infection · 2020Article
- Atypical bacterial pneumonia in the HIV-infected population.Pneumonia (Nathan Qld.) · 2017Review
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
Abstract
Legionnaires' disease is a severe respiratory disease that is estimated to cause between 8,000 and 18,000 hospitalizations each year, though the exact burden is unknown due to under-utilization of diagnostic testing. Although Legionella pneumophila is the most common species detected in clinical cases (80-90%), other species have also been reported to cause disease. However, little is known about Legionnaires' disease caused by these non-pneumophila species. We designed a multiplex real-time PCR assay for detection of all Legionella spp. and simultaneous specific identification of four clinically-relevant Legionella species, L. anisa, L. bozemanii, L. longbeachae, and L. micdadei, using 5'-hydrolysis probe real-time PCR. The analytical sensitivity for detection of nucleic acid from each target species was ≤50fg per reaction. We demonstrated the utility of this assay in spiked human sputum specimens. This assay could serve as a tool for understanding the scope and impact of non-pneumophila Legionella species in human disease.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.