ArticlePlant molecular biology2016
Characterization of OfWRKY3, a transcription factor that positively regulates the carotenoid cleavage dioxygenase gene OfCCD4 in Osmanthus fragrans.
Article in Plant molecular biology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 48 papers.
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48 citing papers in PubMed, 116 citations in OpenAlex.
- The epigenetics of color swatches: histone deacetylation regulates green coloration in Chrysanthemum.The Plant cell · 2026Article
- CcWRKY31 Regulates Postharvest Mandarin Fruit Coloration in a Temperature-Dependent Manner.International journal of molecular sciences · 2026Article
- OfWRKY48 coordinates floral aroma biosynthesis by dual regulation of MEP pathway and carotenoid metabolism inHorticulture research · 2026Article
- Regulation of apocarotenoids for quality improvement and biofortification of horticultural crops.Journal of advanced research · 2026Review
- Full-length transcriptome ofPlant biotechnology (Tokyo, Japan) · 2025Article
- SiLRL1, a bHLH transcription factor from foxtail millet, promotes carotenoid accumulation and improves drought tolerance.BMC plant biology · 2025Article
- Cloning and functional analysis of transcription factorPlant signaling & behavior · 2025Article
- Regulation of important natural products biosynthesis by WRKY transcription factors in plants.Journal of advanced research · 2025Review
- WRKYs as regulatory hubs of secondary metabolic networks: Diverse inducers and distinct responses.Plant communications · 2025Review
- OfWRKY33 binds to the promoter of key linalool synthase geneHorticulture research · 2025Article
- Integrated transcriptome and metabolome analyses provide molecular insights into the transition of flower color in the rose cultivar 'Juicy Terrazza'.BMC plant biology · 2025Article
- VviWRKY24 promotesHorticulture research · 2025Article
- Systematic Analysis ofGenes · 2025Article
- Light Spectral-Ranged Specific Metabolisms of Plant Pigments.Metabolites · 2024Review
- Unbalanced Expression of Structural Genes in Carotenoid Pathway Contributes to the Flower Color Formation of theInternational journal of molecular sciences · 2024Article
- Genome-wide association study of carotenoids in maize kernel.The plant genome · 2024Article
- Integrated Metabolomic and Transcriptomic Profiles Provide Insights into the Mechanisms of Anthocyanin and Carotenoid Biosynthesis in Petals ofPlants (Basel, Switzerland) · 2024Article
- Wolfberry genome database: integrated genomic datasets for studying molecular biology.Frontiers in plant science · 2024Article
- Multiomics analysis provides new insights into the regulatory mechanism of carotenoid biosynthesis in yellow peach peel.Molecular horticulture · 2023Article
- Transcriptional deciphering of the metabolic pathways associated with the bioactive ingredients of wolfberry species with different quality characteristics.BMC genomics · 2023Article
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Authors and funding
8 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The sweet osmanthus carotenoid cleavage dioxygenase 4 (OfCCD4) cleaves carotenoids such as β-carotene and zeaxanthin to yield β-ionone. OfCCD4 is a member of the CCD gene family, and its promoter contains a W-box palindrome with two reversely oriented TGAC repeats, which are the proposed binding sites of WRKY transcription factors. We isolated three WRKY cDNAs from the petal of Osmanthus fragrans. One of them, OfWRKY3, encodes a protein containing two WRKY domains and two zinc finger motifs. OfWRKY3 and OfCCD4 had nearly identical expression profile in petals of 'Dangui' and 'Yingui' at different flowering stages and showed similar expression patterns in petals treated by salicylic acid, jasmonic acid and abscisic acid. Activation of OfCCD4pro:GUS by OfWRKY3 was detected in coinfiltrated tobacco leaves and very weak GUS activity was detected in control tissues, indicating that OfWRKY3 can interact with the OfCCD4 promoter. Yeast one-hybrid and electrophoretic mobility shift assay showed that OfWRKY3 was able to bind to the W-box palindrome motif present in the OfCCD4 promoter. These results suggest that OfWRKY3 is a positive regulator of the OfCCD4 gene, and might partly account for the biosynthesis of β-ionone in sweet osmanthus.
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