ArticlePlant cell reports2016
Wax crystal-sparse leaf 3 encoding a β-ketoacyl-CoA reductase is involved in cuticular wax biosynthesis in rice.
Article in Plant cell reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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16 citing papers in PubMed, 48 citations in OpenAlex.
- Structural, genetic, and adaptive basis of superhydrophobicity in rice leaves.Plant physiology · 2026Article
- Integration of Transcriptome and Metabolome Reveals Wax Serves a Key Role in Preventing Leaf Water Loss in Goji (International journal of molecular sciences · 2024Article
- Decoding drought resilience: a comprehensive exploration of the cotton Eceriferum (CER) gene family and its role in stress adaptation.BMC plant biology · 2024Article
- The AP2 Transcription FactorInternational journal of molecular sciences · 2022Article
- Comprehensive Genome-Wide Identification and Expression Profiling ofFrontiers in plant science · 2022Article
- Overexpression of EiKCS confers paraquat-resistance in rice (Oryza sativa L.) by promoting the polyamine pathway.Pest management science · 2022Article
- Epicuticular Wax Rice Mutants Show Reduced Resistance to Rice Water Weevil (Coleoptera: Curculionidae) and Fall Armyworm (Lepidoptera: Noctuidae).Environmental entomology · 2021Article
- Article
- A Nck-associated protein 1-like protein affects drought sensitivity by its involvement in leaf epidermal development and stomatal closure in rice.The Plant journal : for cell and molecular biology · 2019Article
- Transcription FactorFrontiers in plant science · 2019Article
- Transcriptome analysis reveals crucial genes involved in the biosynthesis of nervonic acid in woody Malania oleifera oilseeds.BMC plant biology · 2018Article
- Three endoplasmic reticulum-associated fatty acyl-coenzyme a reductases were involved in the production of primary alcohols in hexaploid wheat (Triticum aestivum L.).BMC plant biology · 2018Article
- The E3 Ligase DROUGHT HYPERSENSITIVE Negatively Regulates Cuticular Wax Biosynthesis by Promoting the Degradation of Transcription Factor ROC4 in Rice.The Plant cell · 2018Article
- Molecular mapping and candidate gene analysis of a new epicuticular wax locus in sorghum (Sorghum bicolor L. Moench).TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2017Article
- A β-Ketoacyl-CoA Synthase Is Involved in Rice Leaf Cuticular Wax Synthesis and Requires a CER2-LIKE Protein as a Cofactor.Plant physiology · 2017Article
- Fine-Mapping and Analysis ofFrontiers in plant science · 2017Article
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Authors and funding
16 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
key messageWSL3 encodes β-ketoacyl-CoA reductase (KCR) in rice, in a similar way to YBR159w in yeast, and is essential for VLCFA biosynthesis and leaf wax accumulation. Cuticular waxes on plant surfaces limit non-stomatal water loss, protect plants against deposits of dust and impose a physical barrier to pathogen infection. We identified a wax-deficient mutant of rice, wax crystal-sparse leaf 3 (wsl3), which exhibits a pleiotropic phenotype that includes reduced epicuticular wax crystals on the leaf surface and altered wax composition. Map-based cloning demonstrated that defects in the mutant were caused by two adjacent single-nucleotide changes in a gene encoding β-ketoacyl-CoA reductase (KCR) that catalyzes the second step of the fatty acid elongation reaction. The identity of WSL3 was further confirmed by genetic complementation. Transient assays of fluorescent protein-tagged WSL3 in tobacco protoplasts showed that WSL3 localizes to the endoplasmic reticulum, the compartment of fatty acid elongation in cells. Quantitative PCR and histochemical staining indicated that WSL3 is universally expressed in tissues. RNA interference of WSL3 caused a phenotype that mimicked the wsl3 mutant. Very long-chain fatty acids (VLCFAs) 20:0 and 22:0, or 20:1Δ(11) and 22:1Δ(13), were detected when WSL3 and Arabidopsis fatty acid elongation 1 (FAE1) were co-expressed in a yeast ybr159wΔ mutant strain. Our results indicated that WSL3 affects rice cuticular wax production by participating in VLCFA elongation.
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