Evidence map›Paper›PMID 27105387›Full record

ArticleEndocrinology2016

Development and Characterization of Novel Rat Anti-mERβ Sera.

Horacio J Novaira, J B Graceli, S Capellino, A Schoeffield, G E Hoffman, A Wolfe, F Wondisford, S Radovick

Open access · bronzeAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Endocrinology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
0.3field-weighted citation impact, top 27% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 2 citations in OpenAlex.

  1. Article
  2. Impairments in the reproductive axis of female mice lacking estrogen receptor β in GnRH neurons.American journal of physiology. Endocrinology and metabolism · 2018
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 7 institutions in 3 countries.

Horacio J NovairaDepartment of Pediatrics (H.J.N., S.R.), Division of Endocrinology, and Department of Medicine (F.W.), Rutgers Robert Wood Johnson Medical School, New Brunswick, New Jersey 08901; Department of Morphology (J.B.G.), Federal University of Espirito Santo, Vitoria 29040090, Brazil; University of Gießen and Kerckhoff Klinik Bad Nauheim (S.C.), Hessen DE-61231, Germany; Biology Department (A.S.), Loyola University, Baltimore, Maryland 21210; Department of Biology (G.E.H.), Morgan State University, Baltimore, Maryland 21251; and Department of Pediatrics (A.W.), Division of Endocrinology, Johns Hopkins University School of Medicine, Baltimore, Maryland 21287.
J B GraceliDepartment of Pediatrics (H.J.N., S.R.), Division of Endocrinology, and Department of Medicine (F.W.), Rutgers Robert Wood Johnson Medical School, New Brunswick, New Jersey 08901; Department of Morphology (J.B.G.), Federal University of Espirito Santo, Vitoria 29040090, Brazil; University of Gießen and Kerckhoff Klinik Bad Nauheim (S.C.), Hessen DE-61231, Germany; Biology Department (A.S.), Loyola University, Baltimore, Maryland 21210; Department of Biology (G.E.H.), Morgan State University, Baltimore, Maryland 21251; and Department of Pediatrics (A.W.), Division of Endocrinology, Johns Hopkins University School of Medicine, Baltimore, Maryland 21287.
S CapellinoDepartment of Pediatrics (H.J.N., S.R.), Division of Endocrinology, and Department of Medicine (F.W.), Rutgers Robert Wood Johnson Medical School, New Brunswick, New Jersey 08901; Department of Morphology (J.B.G.), Federal University of Espirito Santo, Vitoria 29040090, Brazil; University of Gießen and Kerckhoff Klinik Bad Nauheim (S.C.), Hessen DE-61231, Germany; Biology Department (A.S.), Loyola University, Baltimore, Maryland 21210; Department of Biology (G.E.H.), Morgan State University, Baltimore, Maryland 21251; and Department of Pediatrics (A.W.), Division of Endocrinology, Johns Hopkins University School of Medicine, Baltimore, Maryland 21287.
A SchoeffieldDepartment of Pediatrics (H.J.N., S.R.), Division of Endocrinology, and Department of Medicine (F.W.), Rutgers Robert Wood Johnson Medical School, New Brunswick, New Jersey 08901; Department of Morphology (J.B.G.), Federal University of Espirito Santo, Vitoria 29040090, Brazil; University of Gießen and Kerckhoff Klinik Bad Nauheim (S.C.), Hessen DE-61231, Germany; Biology Department (A.S.), Loyola University, Baltimore, Maryland 21210; Department of Biology (G.E.H.), Morgan State University, Baltimore, Maryland 21251; and Department of Pediatrics (A.W.), Division of Endocrinology, Johns Hopkins University School of Medicine, Baltimore, Maryland 21287.
G E HoffmanDepartment of Pediatrics (H.J.N., S.R.), Division of Endocrinology, and Department of Medicine (F.W.), Rutgers Robert Wood Johnson Medical School, New Brunswick, New Jersey 08901; Department of Morphology (J.B.G.), Federal University of Espirito Santo, Vitoria 29040090, Brazil; University of Gießen and Kerckhoff Klinik Bad Nauheim (S.C.), Hessen DE-61231, Germany; Biology Department (A.S.), Loyola University, Baltimore, Maryland 21210; Department of Biology (G.E.H.), Morgan State University, Baltimore, Maryland 21251; and Department of Pediatrics (A.W.), Division of Endocrinology, Johns Hopkins University School of Medicine, Baltimore, Maryland 21287.
A WolfeDepartment of Pediatrics (H.J.N., S.R.), Division of Endocrinology, and Department of Medicine (F.W.), Rutgers Robert Wood Johnson Medical School, New Brunswick, New Jersey 08901; Department of Morphology (J.B.G.), Federal University of Espirito Santo, Vitoria 29040090, Brazil; University of Gießen and Kerckhoff Klinik Bad Nauheim (S.C.), Hessen DE-61231, Germany; Biology Department (A.S.), Loyola University, Baltimore, Maryland 21210; Department of Biology (G.E.H.), Morgan State University, Baltimore, Maryland 21251; and Department of Pediatrics (A.W.), Division of Endocrinology, Johns Hopkins University School of Medicine, Baltimore, Maryland 21287.
F WondisfordDepartment of Pediatrics (H.J.N., S.R.), Division of Endocrinology, and Department of Medicine (F.W.), Rutgers Robert Wood Johnson Medical School, New Brunswick, New Jersey 08901; Department of Morphology (J.B.G.), Federal University of Espirito Santo, Vitoria 29040090, Brazil; University of Gießen and Kerckhoff Klinik Bad Nauheim (S.C.), Hessen DE-61231, Germany; Biology Department (A.S.), Loyola University, Baltimore, Maryland 21210; Department of Biology (G.E.H.), Morgan State University, Baltimore, Maryland 21251; and Department of Pediatrics (A.W.), Division of Endocrinology, Johns Hopkins University School of Medicine, Baltimore, Maryland 21287.
S RadovickDepartment of Pediatrics (H.J.N., S.R.), Division of Endocrinology, and Department of Medicine (F.W.), Rutgers Robert Wood Johnson Medical School, New Brunswick, New Jersey 08901; Department of Morphology (J.B.G.), Federal University of Espirito Santo, Vitoria 29040090, Brazil; University of Gießen and Kerckhoff Klinik Bad Nauheim (S.C.), Hessen DE-61231, Germany; Biology Department (A.S.), Loyola University, Baltimore, Maryland 21210; Department of Biology (G.E.H.), Morgan State University, Baltimore, Maryland 21251; and Department of Pediatrics (A.W.), Division of Endocrinology, Johns Hopkins University School of Medicine, Baltimore, Maryland 21287.
Johnson University · USJohns Hopkins University · USLoyola University Maryland · USMorgan State University · USRutgers, The State University of New Jersey · USUniversidade Federal do Espírito Santo · BRUniversity of Giessen · DE

Funding

Sex Steroids, Kisspeptin and Regulation of GnRHR01HD068777 · NICHD · RBHS-ROBERT WOOD JOHNSON MEDICAL SCHOOL · PI HOFFMAN, GLORIA E, LEVINE, JON E · 2012 to 2016
$2.4M
1/2 Kisspeptin Regulation and GPR54 Signaling in ReproductionU01HD066432 · NICHD · JOHNS HOPKINS UNIVERSITY · PI HOFFMAN, GLORIA E, RADOVICK, SALLY · 2010 to 2014
$1.6M
NICHD NIH HHS R01 HD068777NICHD NIH HHS U01 HD066432
6 · The paper itself

Abstract

Estrogens regulate normal sexual and reproductive development in females. Their actions are mediated mainly by estrogen receptor (ER)α and ERβ. Understanding the function of ERs necessitates knowing their cellular location and protein partners, which, in turn, requires reliable and specific antibodies. Several antibodies are available for ERα; however, discrepancies in immunoreactivity have been reported for ERβ. Here, we have developed antisera for mouse ERβ (mERβ) using a specific C-terminal 18-amino acid peptide conjugated to mariculture keyhole limpet hemocyanin. Sprague Dawley rats were immunized, and the resulting antisera were characterized by Western blot analysis of nuclear extracts from tissues of wild-type (WT) mice, and mice genetically modified to lack either ERα (CERαKO) or ERβ (CERβKO). An approximately 56-kDa protein was detected in the hypothalamus, uterus, ovary, mammary gland, testes, and epididymis of WT mice, consistent with the predicted molecular size of ERβ. In addition, the same protein band was identified in in vitro synthesized mERβ protein and in the mammary glands of CERαKO mice. The approximately 56-kDa protein was not observed in in vitro synthesized mERα protein or in any tissue examined in the CERβKO mice. Immunohistochemistry using the antisera revealed ERβ staining in the granulosa cells of WT ovaries and in the mediobasal hypothalamus, paraventricular nucleus, and cerebral cortex in the WT adult mouse brain. These data suggest that the novel rat anti-mERβ sera are specific to ERβ to allow investigators to explore to cellular and physiological role of ERβ in the brain and other mouse tissues.

Indexed as

Immune SeraAnimalsEpididymisEstrogen Receptor betaFemaleHypothalamusMaleMammary Glands, AnimalMiceOvaryRatsRats, Sprague-DawleyTestisUterusEstrogen Receptor betaImmune Sera

Identifiers

PMID27105387
PMCPMC4929549
OpenAlexW2340099483

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.