ArticlePloS one2016
RNA Interference based Approach to Down Regulate Osmoregulators of Whitefly (Bemisia tabaci): Potential Technology for the Control of Whitefly.
Article in PloS one, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
28 citing papers in PubMed, 77 citations in OpenAlex.
- Efficacy of Select Gene Targets for the Management of Western Flower Thrips Through RNA Interference.Archives of insect biochemistry and physiology · 2026Article
- Basic Research on Whitefly Molecular Biology: A Foundation for Innovative Pest Management Strategies.Current issues in molecular biology · 2026Review
- Nanoclay-Mediated RNA Interference Targeting Insecticide Receptors Enhances Whitefly Mortality and Reduces Fecundity.Molecular biotechnology · 2026Article
- Control of two sucking insect pests, a whitefly (Bemisia tabaci) and a thrips (Frankliniella occidentalis), infesting hot peppers by spraying LDH-formulated dsRNA.Pest management science · 2025Article
- Transgenic tomato strategies targeting whitefly eggs from apoplastic or ovary-directed proteins.BMC plant biology · 2024Article
- Article
- Horizontally transferred genes as RNA interference targets for aphid and whitefly control.Plant biotechnology journal · 2023Article
- Effect of silencingFrontiers in plant science · 2023Article
- Plant resistance against whitefly and its engineering.Frontiers in plant science · 2023Review
- Foliar application of clay-delivered RNA interference for whitefly control.Nature plants · 2022Article
- RNAi Feeding Bioassay: A Protocol for dsRNA Screening Against Asian Citrus Psyllid and Related Hemipteran Insects.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Application progress of plant-mediated RNAi in pest control.Frontiers in bioengineering and biotechnology · 2022Review
- Silencing of vitellogenin gene contributes to the promise of controlling red palm weevil, Rhynchophorus ferrugineus (Olivier).Scientific reports · 2021Article
- Optimizing Efficient RNAi-Mediated Control of Hemipteran Pests (Psyllids, Leafhoppers, Whitefly): Modified Pyrimidines in dsRNA Triggers.Plants (Basel, Switzerland) · 2021Article
- Review
- Target of rapamycin (Journal of biosciences · 2021Article
- RNAi-mediated silencing of vitellogenin gene curtails oogenesis in the almond moth Cadra cautella.PloS one · 2021Article
- Article
- Debugging: Strategies and Considerations for Efficient RNAi-Mediated Control of the WhiteflyInsects · 2020Review
- CRISPR-Cas9-Based Genome Editing in the Silverleaf Whitefly (The CRISPR journal · 2020Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Over the past decade RNA interference (RNAi) technology has emerged as a successful tool not only for functional genomics, but in planta expression of short interfering RNAs (siRNAs) that could offer great potential for insect pest management. The diet of insects feeding exclusively on phloem sieves contains water and sugars as main components, and the uptake of the liquid food greatly depends on the osmotic pressure within the insect body. Based on this physiological mechanism, transgenic plants of Nicotiana tabacum were generated expressing double stranded RNA (dsRNA) against both aquaporin (AQP) and a sucrase gene, alpha glucosidase (AGLU). These two genes are involved in osmotic pressure maintenance particularly in sap sucking insects, and the aim was to disrupt osmoregulation within the insect ultimately leading to mortality. Real time quantitative PCR (RT-qPCR) was performed to assess the suppression of gene expression in Bemisia tabaci (B. tabaci) and mortality was recorded during transgenic tobacco feeding bioassays. Feeding of insects on plants expressing dsRNA significantly reduced the transcript level of the target genes in B. tabaci after six days of feeding and more than 70% mortality was observed in B. tabaci fed on transgenic plants compared to the control plants. Our data shows that down-regulation of genes related to osmoregulation may find practical applications for the control of this important pest in cotton and other crops.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.