ArticleMicrobiology insights2016
Development of Candida-Specific Real-Time PCR Assays for the Detection and Identification of Eight Medically Important Candida Species.
Article in Microbiology insights, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers, 1 of them a synthesis that pooled it.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
33 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Nucleic Acid Amplification Tests forPathogens (Basel, Switzerland) · 2026Pooled it
- Persistent, Asymptomatic Colonization withMicrobiology spectrum · 2022Trial
- Optimized Selective Media Enhance the Isolation and Characterization of Gut-Derived Probiotic Yeasts.Journal of fungi (Basel, Switzerland) · 2025Article
- Neonatal fungi promote lifelong metabolic health through macrophage-dependent β cell development.Science (New York, N.Y.) · 2025Article
- Biodetection Strategies for Selective Identification of Candidiasis.Indian journal of microbiology · 2024Review
- Investigation of adhesion status of Candida species to the surface of resin materials produced at different angles with additive manufacturing.BMC oral health · 2024Article
- Co-colonization of methicillin-resistant Staphylococcus aureus and Candida spp. in children with malignancies.AMB Express · 2024Article
- Dysbiosis of the intestinal fungal microbiota increases lung resident group 2 innate lymphoid cells and is associated with enhanced asthma severity in mice and humans.Respiratory research · 2023Article
- Candida albicans-specific Th17 cell-mediated response contributes to alcohol-associated liver disease.Cell host & microbe · 2023Article
- In Search for Reasons behindJournal of fungi (Basel, Switzerland) · 2023Article
- Diagnosis of OralArchives of Razi Institute · 2023Article
- Rapid preparation of Candida genomic DNA: combined use of enzymatic digestion and thermal disruption.AMB Express · 2023Article
- Comparative Analysis of Cutaneous Fungi in Atopic Dermatitis Patients and Healthy Individuals.Annals of dermatology · 2022Article
- Gut- and oral-dysbiosis differentially impact spinal- and bulbar-onset ALS, predicting ALS severity and potentially determining the location of disease onset.BMC neurology · 2022Article
- Commensal bacteria and fungi differentially regulate tumor responses to radiation therapy.Cancer cell · 2021Article
- Fluorescent toys 'n' tools lighting the way in fungal research.FEMS microbiology reviews · 2021Article
- Evaluation of 11 DNA Automated Extraction Protocols for the Detection of the 5 Mains Candida Species from Artificially Spiked Blood.Journal of fungi (Basel, Switzerland) · 2021Article
- Intestinal fungi are causally implicated in microbiome assembly and immune development in mice.Nature communications · 2020Article
- Associations between the vaginal microbiome and Candida colonization in women of reproductive age.American journal of obstetrics and gynecology · 2020Article
- Translation elongation factor 1-alpha gene as a marker for diagnosing of candidal onychomycosis.Current medical mycology · 2020Article
Corrections and comments
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Culture-based identification methods have been the gold standard for the diagnosis of fungal infection. Currently, molecular technologies such as real-time PCR assays with short turnaround time can provide desirable alternatives for the rapid detection of Candida microbes. However, most of the published PCR primer sets are not Candida specific and likely to amplify DNA from common environmental contaminants, such as Aspergillus microbes. In this study, we designed pan-Candida primer sets based on the ribosomal DNA-coding regions conserved within Candida but distinct from those of Aspergillus and Penicillium. We demonstrate that the final two selected pan-Candida primer sets would not amplify Aspergillus DNA and could be used to differentiate eight medically important Candida pathogens in real-time PCR assays based on their melting profiles, with a sensitivity of detection as low as 10 fg of Candida genomic DNA. Moreover, we further evaluated and selected species-specific primer sets covering Candida albicans, Candida glabrata, Candida tropicalis, and Candida dubliniensis and show that they had high sensitivity and specificity. These real-time PCR primer sets could potentially be assembled into a single PCR array for the rapid detection of Candida species in various clinical settings, such as corneal transplantation.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.