ArticleScientific reports2016
Crystal Structures and Binding Dynamics of Odorant-Binding Protein 3 from two aphid species Megoura viciae and Nasonovia ribisnigri.
Article in Scientific reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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24 citing papers in PubMed, 75 citations in OpenAlex.
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- Expression and purification of Drosophila OBP44a with the aids of LC-MS and NMR.Protein expression and purification · 2023Article
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- Roles of insect odorant binding proteins in communication and xenobiotic adaptation.Frontiers in insect science · 2023Review
- Differentiation of action mechanisms between natural and synthetic repellents through neuronal electroantennogram and proteomic in Aedes aegypti (Diptera: Culicidae).Scientific reports · 2022Article
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- Chemosensory protein 4 is required forFrontiers in physiology · 2022Article
- Binding Affinity Characterization of Four Antennae-Enriched Odorant-Binding Proteins FromFrontiers in physiology · 2022Article
- Functional Characterization of Odorant Binding Protein PyasOBP2 From the Jujube Bud Weevil,Frontiers in physiology · 2022Article
- Insights Into Chemosensory Proteins From Non-Model Insects: Advances and Perspectives in the Context of Pest Management.Frontiers in physiology · 2022Review
- Function and Characterization Analysis of BodoOBP8 fromInsects · 2021Article
- Identification and Behavioral Assays of Alarm Pheromone in the Vetch Aphid Megoura viciae.Journal of chemical ecology · 2021Article
- Design of a Repellent Against Aedes aegypti (Diptera: Culicidae) Using in silico Simulations With AaegOBP1 Protein.Journal of medical entomology · 2020Article
- Characterization of MaltOBP1, a Minus-C Odorant-Binding Protein, From the Japanese Pine Sawyer Beetle,Frontiers in physiology · 2020Article
- Integrative transcriptomic and genomic analysis of odorant binding proteins and chemosensory proteins in aphids.Insect molecular biology · 2019Article
- Bioinformatic and biochemical analysis of the key binding sites of the pheromone binding protein ofPeerJ · 2019Article
- Protein- and Peptide-Based Biosensors in Artificial Olfaction.Trends in biotechnology · 2018Review
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11 authors at 4 institutions in 3 countries.
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Abstract
Aphids use chemical cues to locate hosts and find mates. The vetch aphid Megoura viciae feeds exclusively on the Fabaceae, whereas the currant-lettuce aphid Nasonovia ribisnigri alternates hosts between the Grossulariaceae and Asteraceae. Both species use alarm pheromones to warn of dangers. For N. ribisnigri this pheromone is a single component (E)-β-farnesene but M. viciae uses a mixture of (E)-β-farnesene, (-)-α-pinene, β-pinene, and limonene. Odorant-binding proteins (OBP) are believed to capture and transport such semiochemicals to their receptors. Here, we report the first aphid OBP crystal structures and examine their molecular interactions with the alarm pheromone components. Our study reveals some unique structural features: 1) the lack of an internal ligand binding site; 2) a striking groove in the surface of the proteins as a putative binding site; 3) the N-terminus rather than the C-terminus occupies the site closing off the conventional OBP pocket. The results from fluorescent binding assays, molecular docking and dynamics demonstrate that OBP3 from M. viciae can bind to all four alarm pheromone components and the differential ligand binding between these very similar OBP3s from the two aphid species is determined mainly by the direct π-π interactions between ligands and the aromatic residues of OBP3s in the binding pocket.
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